大肠杆菌酶基因缺失菌株碳中心代谢系统的代谢通量分析  

FBA and FVA analysis of central carbon metabolism in enzyme deletion mutants of E.coli

作　　者：芮斌[1] 金杰军[1] 范亚东[1] 文汉[1] 

机构地区：[1]安徽农业大学生命科学学院,合肥230036

出　　处：《华中师范大学学报（自然科学版）》2014年第2期251-256,共6页Journal of Central China Normal University：Natural Sciences

基　　金：国家自然科学基金项目(31200626)

摘　　要：大肠杆菌(E.coli)因其遗传信息简单且容易培养而被广泛用作各种生物化学、生物技术研究的模式微生物.为了认识重要模式生物大肠杆菌的代谢系统中各种酶的作用,以软件分析的模式,对已经构建好的小型大肠杆菌代谢系统在cobra中进行代谢通量平衡分析FBA(Flux-Balance Analysis)和代谢通量变化性分析FVA(Flux Variability Analysis),分别单独敲除代谢系统中的25个酶基因,分析敲除各个酶基因后的FBA和FVA的计算结果,通过作图显示出在大肠杆菌代谢系统中不同酶基因被敲除后每个反应的两项结果变化和不同酶基因被敲除后全部反应的两项结果的变化,以此来推测大肠杆菌代谢系统中各种酶所起的作用.E.coli＇s genetic information is clear,and E.coli is easy to be cultured,so it has been widely used as a model microorganism in various biochemical and biological research.In order to recognize the role of the various enzymes played in E.coli metabolic system,in this thesis FBA（Flux Balance Analysis） and FVA（Fux Variability Analysis）are analyzed by software mode in the cobra of the built small F.coli metabolic system.25 enzymes in the metabolic system separately knockout,FBA and FVA results of the knockout enzymes genes are shown.And through an intuitive graphical comparison of FVA and FVA,it shows that a variety of enzyme gene knock-out effects on both each reaction and all reaction of the E.coli metabolic system.Thus to infer the role played by the various enzymes in the E.coli metabolic system.

关 键 词：代谢通量平衡分析 代谢通量变化性分析 代谢通量 酶基因 

分 类 号：Q55[生物学—生物化学]