乌梅等20种中药对胸膜肺炎放线杆菌的体外抗菌活性研究  

A study on antibacterial activity of crude extract from twenty traditional Chinese medicines like Fructus mume against Actinobacillus pleuropneumoniae in vitro

作　　者：康帅[1] 殷中琼[1] 贾仁勇[1,2] 代如意[1] 李莉[1] 彭练慈[1] 曲径[1] 梁陈[1] 

机构地区：[1]四川农业大学动物医学院,四川雅安625014 [2]四川农业大学预防兽医研究所,四川雅安625014

出　　处：《华南农业大学学报》2014年第3期13-17,共5页Journal of South China Agricultural University

基　　金："十二五"农村领域国家科技计划项目(2011BAD34B03);四川省青年科技创新研究团队(2013TD0015)

摘　　要：【目的】观察乌梅Fructus mume等20种中药的体外抗胸膜肺炎放线杆菌Actinobacillus pleuropneumoniae活性．【方法】通过乙醇回流、水煎煮和超声波等方法对20种中药进行提取，采用二倍稀释法测定其对胸膜肺炎放线杆菌的体外抗菌活性；并研究抗菌活性较强的几味中药的体外联合抑菌活性．【结果和结论】乌梅、黄连Rhizoma cop-tidis、诃子Terminalia chebula、秦皮Cortex fraxini、地榆Sanguisorbae officinalis、虎杖Polygonum cuspidatum 6种中药提取物对胸膜肺炎放线杆菌的最小抑菌浓度（ Minimal inhibitory concentration ，MIC）范围是6.25～50.00 mg/mL；大青叶Isatis indigotica、茵陈Artemisia capillaris、甘草Glycyrrhiza uralensis和白鲜皮Dictamnus dasycarpus 4种中药提取物的MIC范围是50.00～100.00 mg/mL；黄柏Phellodendron amurense、杜仲Eucommia ulmoides、金银花Lonicera japoni-ca、柴胡Bupleurum chinense、蒲公英Taraxacum mongolicum、板蓝根Baphicacanthus cusia、栀子Gardenia jasminoides和黄芪Astragalus membranaceus等10种中药提取物的MIC＞100.00 mg/mL．联合抑菌试验结果表明，乌梅、黄连、诃子和虎杖两两联合抑菌指数（FICI）≤1，乌梅、虎杖分别与秦皮的FICI＞2．乌梅、黄连、诃子、虎杖、秦皮、地榆对胸膜肺炎放线杆菌均具有较好的体外抗菌活性；乌梅、黄连、诃子和虎杖两两联合为相加、协同作用；秦皮分别与乌梅、虎杖为拮抗作用．[Objective]The antibacterial activity of twenty traditional Chinese medicines against Actinoba-cillus pleuropneumoniae in vitro was evaluated in this study .[Method]Twenty traditional Chinese medi-cines like Fructus mume were extracted by ethanol reflux extraction ,water decoction and ultrasonic wave . Minimal inhibitory concentrations （ MICs） of twenty traditional Chinese medicines against A.pleuropneu-moniae were determined by the macrodilution broth method .The joint antimicrobial activity of some tradi-tional Chinese medicines was evaluated in vitro.[Result and conclusion]The results showed that the MICs of the extract obtained from F.mume, Rhizoma coptidis, Terminalia chebula, Cortex fraxini, Sanguisorbae officinalis and Polygonum cuspidatum against A.pleuropneumoniae ranged from 6.25 to 50.00 mg/mL. MICs of the extract obtained from Isatis indigotica, Artemisia capillaris,Glycyrrhiza uralensis and Dictam-nus dasycarpus ranged from 50.00 to 100.00 mg/mL.MICs of the extract obtained from Phellodendron amurense, Eucommia ulmoides, Lonicera japonica, Bupleurum chinense, Taraxacum mongolicum, Baphi-cacanthus cusia, Gardenia jasminoides and Astragalus membranaceus were higher than 100.00 mg/mL. The results of joint bacteriostatic test showed that the fractional inhibitory concentration index （ FICI） of the pairwise combination of F.mume, R.coptidis, Te.chebula and Po.cuspidatum was lower than or equal to 1, and FICI of C.fraxini with Po.cuspidatum and F.mume was higher than 2.The F.mume, R.coptidis, Te.chebula, Po.cuspidatum, C.fraxini and S.officinalis had a good antimicrobial activi-ty.The pairwise combination of F.mume, R.coptidis, Te.chebula and Po.cuspidatum showed an en-hanced or additive synergy action , and C.fraxini showed antagonistic action with F.mume and Po.cus-pidatum, respectively .

关 键 词：乌梅 黄连 诃子 虎杖 胸膜肺炎放线杆菌 抗菌活性 

分 类 号：S852.6[农业科学—基础兽医学]