牛角藓Cratoneuron filicinum (Hedw.) Spruce多样性的SRAP标记研究  

SRAP Markers of Cratoneuron filicinum(Hedw.)Spruce Diversity

作　　者：赵红燕[1] 胡忠健[1] 王挺杨 吴玉环[1,2] 

机构地区：[1]杭州师范大学生命与环境科学学院,浙江杭州310036 [2]中国科学院沈阳应用生态研究所,辽宁沈阳110016

出　　处：《杭州师范大学学报（自然科学版）》2014年第2期153-158,共6页Journal of Hangzhou Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(30970188);浙江省重点科技创新团队基金项目(2010R50039);杭州师范大学科研启动项目(YS05203130)

摘　　要：利用SRAP分子标记方法对不同地区的13份牛角藓Cratoneuron filicinum(Hedw.)Spruce样本进行遗传多样性分析.结果表明,稳定且重复性高的15对引物共扩增出162条清晰的条带,其中161条具有多态性,平均多态性位点比率为99.49%,说明牛角藓的遗传变异丰富.运用PopGen-32软件分析发现,平均等位基因数Na=1.9938,有效等位基因数Ne=1.6248,Neis基因多样性指数H=0.3606,Shannons信息指数I=0.5366,遗传分化系数Gst为0.5736,说明牛角藓的遗传变异主要在居群之间.通过NTSYS2.1软件对14份样本的亲缘关系用UPG-MA方法进行聚类,样本的遗传距离与地理位置没有相关性.The genetic diversity of 13 samples of Cratoneuron f ilicinum（Hedw .） Spruce collected in different regions in China is analyzed by SRAP molecular marker technique .The results show that 15 stable and highly reproducible primers amplify 162 polymorphic bands ,161 bands have polymorphism ,and the average percentage of polymorphism reaches to 99 . 49% ,which indicate the rich genetic variation of Cratoneuron f ilicinum .Using the software POPGENE-32 ,it shows that the average allele number Na is 1 .9938 ,the effective allele number Ne is 1 .6248 ,Nei＇s gene diversity index H is 0 .3606 , Shannon＇s information index is 0 .5366 ,and the genetic differentiation coefficient is 0 .5736 ,these state that the genetic variation of Cratoneuron f ilicinum mainly occurs among populations .The cluster analysis on the genetic relationships among 14 samples by UPG-MA method with the software NTSYS2 .1 shows that there is no correlation between the genetic distance and geographic distance of the samples .

关 键 词：牛角藓 SRAP 遗传多样性 

分 类 号：Q949[生物学—植物学]