白细胞介素10在支气管哮喘发病机制中的作用研究  

Interleukin 10 in the pathogenesis of asthma

作　　者：喻昌利[1] 郭艳丽[1] 郝小惠[2] 刘和亮[2] 王红阳[1] 张芳[1] 

机构地区：[1]河北联合大学附属医院,河北唐山063000 [2]河北联合大学医学实验研究中心,河北唐山063000

出　　处：《中国现代医学杂志》2014年第2期19-23,共5页China Journal of Modern Medicine

基　　金：河北省自然科学基金(No:C2010001788)

摘　　要：目的研究白细胞介素10在支气管哮喘发病机制中的作用。方法哮喘组用卵白蛋白OVA敏原复制C57小鼠哮喘模型;IL-10抗体干预组与哮喘组均在激发后24、48和96 h及第8天行支气管肺泡灌洗(BAL),酶联免疫吸附法(ELISA)测定BALF中IL-10、IL-12、TNF-α及血清中IgE、一氧化氮(NO)浓度。结果哮喘模型组IL-10抗体干预组BALF中TNF-α和血清中IgE、NO含量显著高于对照组,BALF中IL-10、IL-12含量显著低于对照组。哮喘组和IL-10抗体干预组内IL-10与IL-12均呈正相关;哮喘组IL-10与TNF-α、IgE呈负相关;IL-10抗体干预组内IL-10浓度与相应时相TNF-α、IgE、NO浓度做直线回归分析,显示负相关关系。结论 IL-10可能参与支气管哮喘发病的整个过程,随着IL-10含量的减少,支气管哮喘进行性加重。因此在支气管哮喘不同时期检测IL-10水平具有重要的临床意义。[Objective] To study the role of interleukin 10 in the pathogenesis of bronchial asthma. [Methods] Asthma group with ovalbumin OVA allergen preparation C57 mice model of asthma, antibody IL-10 intervention group and asthma group were 24 h, 48 h, 96 h after excitation, and 8D underwent bronchoalveolar lavage （BAL）, enzyme linked immunosorbent assay （ELISA） in BALF by IL- 10, IL- 12, TNF -ct and IgE in serum, the concentration of nitric oxide. [Results] IgE, the content of NO in serum TNF- α and BALF in asthma model group and intervention group was significantly higher than that of the control group. Asthma group and intervention group in both IL-10 and IL-12 positive correlation, a negative correlation between IL- 10 and TNF-α in asthmatic group, IgE. Concentration of IL- 10 antibody intervention group and IL- 10 concentration of TNF-α, IgE, NO in linear regression analysis, showed negative correlation. [Conclusion] IL- 10 may be is involved in the pathogenesis of bronchial asthma throughout the process, with reduced IL-10 content, bronchial asthma aggravated sexual. Therefore in bronchial asthma in different periods to detect IL-10 levels has important clinical significance.

关 键 词：支气管哮喘 IL-10 IGE NO 

分 类 号：R562.25[医药卫生—呼吸系统]