ADAMTS-1对小鼠肺成纤维细胞Ⅰ型胶原蛋白的影响  

ADAMTS-1 influence expression of type 1 collagen in vitro

作　　者：董素素[1] 张平[1] 许霞辉[1] 阳洁[1] 

机构地区：[1]南华大学第一附属医院呼吸内科,湖南衡阳421001

出　　处：《中国现代医学杂志》2014年第2期40-43,共4页China Journal of Modern Medicine

基　　金：湖南省教育厅科研基金(No:10A107)

摘　　要：目的探讨含Ⅰ型血小板结合蛋白基序的解聚蛋白样金属蛋白酶(ADAMTS-1)对小鼠肺成纤维细胞Ⅰ型胶原蛋白(COL1)表达的影响及可能的机制。方法应用细胞培养技术进行小鼠肺成纤维细胞培养,利用ADAMTS-1为刺激因子,将培养的细胞分为空白组、低、中、高浓度组,应用逆转录-多聚酶链反应技术(RT-PCR)观察COL1 mRNA、转化生长因子β1(TGF-β1)mRNA在各组中的表达情况;采用蛋白质印迹法(Western blotting)观察各组细胞COL1、TGF-β1的变化。结果与空白组相比,低浓度组、中浓度组、高浓度组的COL1、TGF-β1表达水平下降,其中高浓度组表达水平最低(P<0.05)。结论 ADAMTS-1能降低COL1的表达,抑制TGF-β1是其抗纤维化作用的可能潜在机制。[Objective] To explore the influence of ADAMTS- 1 on expression of collagen I in vitro and its possible mechanism. [Methods] Mouse lung fibroblasts were cuhured. They were assigned to tbre groups： normal control group, low concentration group, medium concentration group, high concentration group. Type I collagen and TGF--β, were measured by RT-PCR and western blotting. [Results] Compared with normal control group, the levels of Type 1 collagen and TGF --β1 were significantly decreased in other three groups, especially in high concentration group （P〈0.05）. [Conclusion] ADAMTS-I can degrade Type I colla- gen, inhibiting TGF--β1 may be a potential mechanism of its anti-fibrosis.

关 键 词：含Ⅰ型血小板结合蛋白基序的解聚蛋白样金属蛋白酶 肺成纤维细胞 Ⅰ型胶原蛋白 转化生长因子Β1 

分 类 号：R563[医药卫生—呼吸系统]