HPLC波长切换法测定青阳参中7个C_(21)甾体皂苷的含量  

Simultaneous determination of seven C_(21) steroidal saponins in the radix of Cynanchum otophyllum Schneid. by HPLC wavelength switching method

作　　者：杨金铧 殷敏敏[1] 张健[2] 王磊[3] 叶文才[3] 殷志琦[1] 

机构地区：[1]中国药科大学天然药物化学教研室&天然药物活性组分与药效国家重点实验室,南京210009 [2]江苏省中医药研究院转化医学实验室,南京210028 [3]暨南大学中药及天然药物研究所&中药药效物质基础及创新药物研究&广东省高校重点实验室,广州510632

出　　处：《中国药科大学学报》2014年第2期200-204,共5页Journal of China Pharmaceutical University

基　　金：国家自然科学基金资助项目(No.81001379);天然药物活性组分与药效国家重点实验室资助项目(No.ZJ11175);江苏省第九批"六大人才高峰"高层次人才项目(No.2012-YY-008);江苏省"青蓝工程"资助项目~~

摘　　要：建立HPLC波长切换法，同时测定青阳参中7个C21甾体皂苷的含量。采用Alhima C18（250mm×4．6mm，5μm）色谱柱，以乙腈-水为流动相，梯度洗脱，流速为1．0mlMmin，柱温为35℃，检测波长为259nm（0～27min），223nm（27～72min）。结果表明，该7个C21甾体皂苷在各自检测范围内线性良好，加样回收率范围在98．5％-103．9％之间。通过对不同产地的7个批次青阳参中7个成分的含量测定，认为该方法简便、准确、快速，可用于青阳参药材的质量控制。An HPLC method was established to evaluate the quality of Cynanchum otophyllum with a simultaneous determination of seven C21 steroidal saponins. The method was carried out on an Alltima C18 column （250 mm × 4.6 mm, 5 μm） with a mobile phase consisting of acetonitrile-water at the flow rate of 1.0 mL/min. The detection wavelength was 259 nm （0-27 min）, 223 nm （27-72 min） and column temperature was 35 ℃. The seven compounds were completely separated with good linearity. The average recoveries varied from 98.5% to 103.9%. These results indicated that the method was convenient, accurate and rapid for the quality control of Radix of Cynartchum otophyllum in 7 commercial samples.

关 键 词：青阳参 高效液相色谱 C21甾体皂苷 含量测定 

分 类 号：R284[医药卫生—中药学]