当归芍药散HPLC-DAD指纹图谱峰来源分析  

HPLC-DAD fingerprint of Danggui-Shaoyao-San and its synergy

作　　者：胡虒尧 周瑜[1] 陶春蕾[1] 宋欣 许钒[1] 王运来[1] 

机构地区：[1]安徽中医药大学药学院,合肥230038 [2]安徽万邦医药科技有限公司,合肥230088

出　　处：《中国药科大学学报》2014年第2期205-209,共5页Journal of China Pharmaceutical University

基　　金：国家自然科学基金资助项目(No.81173368)~~

摘　　要：建立了当归芍药散的HPLC-DAD指纹图谱分析方法,确定了部分特征峰的归属。采用高效液相色谱法,色谱柱为Welchrom C18柱(250 mm×4.6 mm,5μm);流动相为乙腈-0.05%磷酸水溶液(梯度洗脱),检测波长为230 nm,流速为1 mL/min。从建立的当归芍药散指纹图谱中,对照出当归芍药散中的21个特征峰8个来自当归,11个来自白芍,13个来自川芎,4个来自白术,2个来自茯苓,3个来自泽泻,同时鉴定出7个峰的化学结构。当归芍药散指纹图谱的结果为鉴别当归芍药散的质量提供了依据,也为阐明各部位的药效物质基础提供了参考。An HPLC-DAD fingerprint method was developed for the simultaneous determination of Danggui- Shaoyao-San. A Welchrom C18 column（250mm×4.6mm,5μm）was used with a gradient mobile phase system of acetonitrile-water containing 0.05% phosphoric acid. The detection wavelength was 230 nm; the flow rate was 1 mL/min. From the fingerprint we can conclude that in the total 21 peaks, 8 was from Angelicae sinensis, 11 from Paeonia lactiflora, 13 from the Ligustici Chuaxiong, 4 from Atractylodis macrocephalae, 2 from Poria cocos, and 3 from Rhizoma Alismatis. The validated methocl was applied to the quality control of Danggui-Shaoyao-San, providing a basis for the efficacy of various parts to clarify the material basis.

关 键 词：当归芍药散 HPLC—DAD 指纹图谱 

分 类 号：R286[医药卫生—中药学]