HPLC法同时测定怀牛膝中3种甾酮类成分  

Simultaneous determination of three sterones in Achyranthis bidentatae Radix by RP-HPLC

作　　者：穆海风 李会军[1] 陈君[1] 李萍[1] 

机构地区：[1]中国药科大学天然药物活性组分与药效国家重点实验室,南京210009

出　　处：《中国药科大学学报》2014年第2期210-212,共3页Journal of China Pharmaceutical University

基　　金：国家中医药管理局中医药行业科研专项资助项目(No.201307002)~~

摘　　要：建立怀牛膝中3种甾酮类成分含量测定方法，比较不同产地牛膝的质量差异。色谱柱为Venusil XBP C18柱，流动相为0．1％甲酸-乙腈（84：16），检测波长为250nm。在所建立的方法中，β-蜕皮甾酮、R-牛膝甾酮和S-牛膝甾酮与杂质分离良好，线性范围分别为3．07—1470．00μg/mL（r=0．9994，n=6），0．52～246．00μg/mL（r=0．9997，n=6），0．47—225．00μg/mL（r=0．9998，n=6），平均回收率分别为101．4％、101．1％和101．1％。本方法适用于同时测定怀牛膝中β-蜕皮甾酮、R-牛膝甾酮及S-牛膝甾酮的含量。An HPLC method was established for the simultaneous determination of β-ecdysterone, R-inokosterone and S-inokosterone in the Achyranthis bidentatae Radix to compare the quality from different habitats. Three phytoecdysones were separated with a Venusil XBP C18 column by isocratic elution using 0. 1% formic acid in water and acetonitrile （84 ： 16） as the mobile phase, while the UV detector wavelength was set at 250 nm. Calibration curves of/3-ecdysterone, R-inokosterone and S-inokosterone were linear over the range of 3.07-1 470. 00, 0. 52-246.00, and 0. 47-225. 00 μg/mL, respectively. Average recoveries were 101.4%, 101.1% and 101.1%, respectively. The established method will be helpful in the quality control of Achyranthis bidentatae Radix.

关 键 词：怀牛膝 含量测定 β-蜕皮甾酮 R-牛膝甾酮 S-牛膝甾酮 

分 类 号：R284.1[医药卫生—中药学]