miRNA-205在猪卵母细胞成熟过程中表达规律及其调控作用的研究  

Stydy on Expression Regulation and Regulatory Functions of miRNA-205 in Pig Oocyte in vitro Maturation

作　　者：陈超[1] 李万宏[1] 孙丽娜[1] 陈树雄[1] 陈璐[1] 李纯锦[1] 周虚[1] 

机构地区：[1]吉林大学动物科学学院,吉林长春130062

出　　处：《中国畜牧兽医》2014年第4期18-22,共5页China Animal Husbandry & Veterinary Medicine

基　　金：国家青年科学基金项目(31301224)

摘　　要：本试验旨在探索miRNA-205在猪卵母细胞成熟过程中表达规律及其调控功能。应用生物学软件TargetScan和microRNA.org对miRNA-205靶基因进行预测,利用实时荧光定量PCR方法分别检测miRNA-205及其靶基因在猪卵丘卵母细胞复合体(cumulus-oocyte complexes,COCs)成熟过程中0、24和48h表达量。结果表明,miRNA-205在猪COCs成熟过程中表达呈上升趋势,0h表达量最低,48h表达量最高。添加FSH、LH和EGF的成熟液组与成熟基液组相比,miRNA-205表达量显著下调,长正五聚蛋白(PTX3)表达量极显著上调(P<0.01),PTX3与miRNA-205的表达量呈负相关,卵丘细胞扩散标记基因肿瘤致死因子(TNFAIP6)和透明质酸合成酶(HAS2)的表达量极显著上调(P<0.01)。由此推断,miRNA-205保守序列可能与PTX3基因3′-UTR区特异性结合参与卵丘细胞扩散的调控。The research aimed to assess the expression profile and regulatory functions of miRNA 205 in pig oocyte in vitro maturation. The potential target of miRNA-205 was predicted by biology software including TargetScan and mieroRNA, org. The expression profile of miRNA-205 and its target gene were detected by Real-time PCR in pig cumulus-oocyte complexes （COCs） in vitro maturation 0, 24 and 48 h. The expression of miRNA-205 in pig COCs maturation gradually increased, 0 h lowest, 48 h maximum. The expression of miRNA-205 was significantly reduced in mature solution added EFG, FSH and LH compared with which in a basic mature solution, the expression of PTX3 and cumulus proliferation-related genes including TN- FAIP6 and HAS2 were significantly increased. The expression of PTX3 was negative correlation with that of miRNA-205 in porcine oocyte maturation. From these results could be concluded that the miRNA-205 conserved sequence specifically bound to 3CUTR region of PTX3 gene might be participated in the regulation of oocyte maturation.

关 键 词：MIRNA 205 卵丘卵母细胞复合体 实时荧光定量PCR 卵母细胞成熟 

分 类 号：Q78[生物学—分子生物学]