6个绵羊品种微卫星多样性分析  

Analysis of Genetic Diversity on Microsatellite in Six Sheep Breeds

作　　者：王亚磊[1,2,3] 李静心[1,3] 茆达干[2] 王慧利[1,3] 李隐侠[1,3] 钱勇[1,3] 孟春花[1,3] 曹少先[1,3] 

机构地区：[1]江苏省农业科学院畜牧研究所,江苏南京210014 [2]南京农业大学动物科技学院,江苏南京210095 [3]江苏省农业科学院动物品种改良和繁育重点实验室,江苏南京210014

出　　处：《中国畜牧兽医》2014年第4期174-179,共6页China Animal Husbandry & Veterinary Medicine

基　　金：江苏省农业自主创新资金(CX(13)2035);公益性行业(农业)科研专项(201303143-06)

摘　　要：为检测兰坪乌骨绵羊的遗传多样性及其与其他5个绵羊品种(湖羊、小尾寒羊、杜泊羊、特克塞尔羊和无角陶赛特羊)的亲缘关系,试验选择9个微卫星位点对共计117个体进行了检测,将各微卫星位点的PCR扩增产物进行非变性聚丙烯酰胺凝胶电泳,POPGEN32软件计算遗传参数。结果表明,9个微卫星位点均表现为高度多态,各绵羊群体内的PIC平均值为湖羊(0.7211)>陶赛特羊(0.6780)>小尾寒羊(0.6492)>乌骨绵羊(0.6479)>特克塞尔羊(0.5930)>杜泊羊(0.5728)。6个品种中乌骨绵羊的平均杂合度最大(0.9543),而杜泊羊最小(0.2556)。UPGMA聚类分析表明,湖羊与小尾寒羊首先聚为一类,乌骨绵羊与湖羊、小尾寒羊遗传距离较近,而与3个引进品种的遗传距离较远。这些结果提示乌骨绵羊可与湖羊或小尾寒羊杂交提高繁殖力,与引进品种杂交提高肉用性能。To detect the genetic diversity of Black bone sheep in Lanping and its genetic relationship with the other five sheep breeds （Hu sheep, Small-tail Han sheep, Dorper sheep, Texel sheep and hornless Dorset sheep）, nine microsatellite loci were selected to study a total of 117 individuals, PCR amplification products of the microsatellite loci were resolved by nativepolyacrylamide gel electrophoresis, and POPGEN32 software was used to calculate genetic parameters. The results showed that nine microsatellite loci were high polymorphism. The mean polymorphism information content （PIC） in each sheep population was 0. 7211 （Hu sheep）, 0. 6780（Dorset sheep）, 0. 6492 （Small-tail Han sheep）, 0. 6479 （Black bone sheep）, 0. 5930 （Texel sheep） and 0. 5728 （Dorper sheep）, respectively. Among the six breeds, the mean heterozygosity （H） of Black bone sheep was highest （0. 9543）, while Dorper sheep was the lowest （0. 2556）. UPGMA cluster analysis showed that Hu sheep and Small-tail Han sheep clustered into a first class; the later was Black bone sheep. The further genetic distance was between Black bone sheep and the three introduced species. These results suggested that Black bone sheep might be crossed with Hu sheep or Small-tail Han sheep to improve fertility, and crossed with the introduced species to improve meat performance.

关 键 词：绵羊 微卫星 遗传多样性 遗传距离 

分 类 号：S813.3[农业科学—畜牧学]