贵州半细毛羊促生长激素分泌素受体基因SNPs筛查与变异分析  

Screening the GHSR Gene for SNPs and Analyzing the Mutations in Guizhou Semi-fine Wool Sheep

作　　者：张琼娣 宋德荣 周大荣 彭华 刘若余[2] 吴蕊汝 江兴美 吴萍 

机构地区：[1]毕节市畜牧兽医科学研究所,贵州毕节551700 [2]贵州大学动物科学学院,贵州花溪550025

出　　处：《中国畜牧兽医》2014年第4期189-193,共5页China Animal Husbandry & Veterinary Medicine

基　　金：国家现代农业产业技术体系专项资金(CARS-40-30;CARS-40-46);贵州省农业动植物育种专项(黔农育专字[2011]016号);毕节地区科技计划项目(毕科合字[2010]05号);贵州省科技厅;毕节地区行署;中科院昆明分院科技合作项目(省地院合2010-05);毕节试验区肉羊产业发展研究人才团队(毕人领办通[2013]7号)

摘　　要：通过研究促生长激素分泌素受体(growth hormone secretagogue receptor,GHSR)基因多态性,为贵州半细毛羊选种选育提供进一步的分子生物学依据。试验选取36只贵州半细毛羊构建DNA池,设计1对引物扩增其GHSR基因第2外显子及第1、2内含子部分序列并进行双向测序,对SNPs位点等位基因频率进行估算,利用在线生物信息学软件分析SNPs位点对GHSR基因RNA二级结构的影响。结果发现,在贵州半细毛羊GHSR基因中筛选到2个SNPs:T70G和G229A,均为同义突变,SNPs位点导致GHSR基因mRNA二级结构发生变化。GHSR基因多态性可能影响贵州半细毛羊的生长。By studying the GHSR gene polymorphism, A basis of selection and breeding was provide on molecular biology of Guizhou Semi-fine Wool sheep. Guizhou Semi-fine Wool sheep were used to construct DNA pools in this study, the complete sequences of exon 2 and part of intron 1 and intron 2 region in GHSR gene were obtained by designing one pair of specific prim- ers. The amplified PCR products would be directly purified and conducted bidirectional sequences subsequently. DNAStar and BLAST software were used to identify polymorphisms of GHSR gene. Moreover, the impact of SNPs on secondary structure of RNA, secondary structure of GHSR protein would be analyzed by the bioinformatics software. The results showed that two SNPs （T70G and G229A） were found in GHSR gene which were two synonymous mutations. The SNPs led to the changes of RNA secondary structure. GHSR gene polymorphism might affect the growth of Guizhou Semi-fine Wool sheep.

关 键 词：贵州半细毛羊 促生长激素分泌素受体基因 SNP DNA池 

分 类 号：S813.3[农业科学—畜牧学]