神经生长因子对小鼠卵巢颗粒细胞增殖的影响  

Effects of Nerve Growth Factor on Proliferation of Mouse Ovarian Granulosa Cells

作　　者：侯晓峰[1] 李万宏[1] 孙丽娜[1] 陈树雄[1] 陈璐[1] 李纯锦[1] 周虚[1] 

机构地区：[1]吉林大学动物科学学院,吉林长春130062

出　　处：《中国畜牧兽医》2014年第4期202-206,共5页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金青年基金项目(31301969)

摘　　要：本研究旨在探索神经生长因子(nerve growth factor,NGF)对小鼠卵巢颗粒细胞增殖的影响。试验建立了小鼠卵巢颗粒细胞的原代培养体系,并通过免疫荧光技术对颗粒细胞进行鉴定和检测NGF及其受体在卵巢颗粒细胞上的表达,采用MTS法检测不同浓度的NGF对昆明小鼠卵巢颗粒细胞增殖的影响。卵巢颗粒细胞在10、50、100、500ng/mL NGF作用24h,用酶标仪测定细胞D490nm值,试验组与对照组相比均有促进增殖的影响(P<0.05),50ng/mL NGF试验组与对照组相比差异极显著(P<0.01)。结果表明,NGF在昆明小鼠的卵巢颗粒细胞中有表达,在一定浓度范围NGF可以促进卵巢颗粒细胞的增殖。To study the effect of nerve growth factor （NGF） on proliferation of mouse ovarian granulosa cells. Primary mouse ovarian granulosa cells were cultured successfully, immunofluorescence was used to identify granulosa cells and observe the distributions of NGF and TrkA in granulosa cells, the MTS was used to analysis the different concentrations of NGF on proliferation of mouse granulosa cells. The cells were treated with NGF of different concentrations （10, 50, 100, 500 ng/mL） for 24 h. The D490 nm value of the cell that was identified was determined by micro-plate reader. Compared with the control group, each treatment group promote the proliferation of granulosa cells with different degrees （P〈0.05）, the effect of NGF group （50 ng/mL） on the granulosa cells proliferation was the most significant （P〈0.01）. These results indicated that NGF could promote proliferation of mouse granulosa cells. Also,mouse ovarian granulosa ceils could express NGF and TrkA.

关 键 词：卵巢颗粒细胞 神经生长因子 增殖 

分 类 号：Q813.11[生物学—生物工程]