反渗透浓缩对四逆汤物料体系理化参数的影响及其相关性研究  

Influence of reverse osmosis concentrate on physicochemical parameters of Sini decoction material system and their relevance

作　　者：金唐慧 张刘红[1] 朱华旭[1,2] 郭立玮[1,2] 李博[1] 鲁明明 

机构地区：[1]南京中医药大学江苏省中药资源产业化过程协同创新中心,江苏南京210046 [2]南京中医药大学江苏省植物药深加工工程研究中心,江苏南京210046 [3]江苏省南星药业有限责任公司,江苏南京210046

出　　处：《中国中药杂志》2014年第8期1421-1425,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30873450,81274096);江苏省科技厅自然科学基金项目(BK2012855);江苏省科技厅产学研联合创新资金--前瞻性研究项目(BY2012036)

摘　　要：目的:通过研究反渗透过程对中药复方物料体系理化参数、渗透压的影响及其相关性,探索复方体系反渗透工艺设计新方法。方法:以四逆汤为模型药物,配成高、中、低3种浓度的物料,分别经5万相对截留分子质量超滤膜预处理后,过反渗透膜,测定各样品的黏度、浊度、电导率、盐度、TDS、pH及渗透压,考察各理化参数之间及其各自与渗透压的相关性。并以HPLC特征图谱考察样品反渗透前后主要化学组成的变化。结果:各样品电导-渗透压、盐度-渗透压、TDS-渗透压3组参数之间的线性相关系数分别为0.963 8,0.932 7,0.973 7;反渗透浓缩液与其超滤液的HPLC特征图谱相似度高达0.968以上(除低浓度组外)。结论:各样品体系的电导、盐度和TDS 3种理化参数与渗透压存在显著的相关性;盐度、电导、TDS三者之间亦显著线性相关。四逆汤物料体系经过反渗透过程后的浓缩液比较完整的保留了原化学组成。Objective： By studying the process of reverse osmosis system for traditional Chinese medicine materials physico- chemical parameters affecting the osmotic pressure of its relevance, new compound system reverse osmosis process design methods were explored. Method： Three concentrations materials for high, middle and low were dubbed with Sini decoction as a model drug, and pretreated by 50 thousand relative molecular weight cut-off ultrafiltration membrane. The viscosity, turbidity, conductivity, salinity, TDS, pH value and osmotic pressure of each sample were determined after the reverse osmosis to study the physical and chemical pa- rameters between their respective correlations with the osmotic pressure, and characterized by HPLC chromatograms showing changes before and after the main chemical composition of samples of reverse osmosis. Result： Conductivity-osmotic pressure, salinity-osmotic pressure of the linear correlation coefficient, TDS-osmotic pressure between the three sets of parameters were 0. 963 8, 0. 932 7, 0. 973 7, respectively. Reverse osmosis concentrate and its characteristic spectrum ultrafiltrate HPLC similarity were up to 0. 968 or more, except the low concentrations. Conclusion： There is a significant correlation between the three physicochemical parameters （conductivity, salinity, TDS） and osmotic pressure of each sample system, and there is also significant linear correlation between sa- linity, conductivity, TDS. The original chemical composition of Sini decoction material concentrate was completely remained after the process of reverse osmosis.

关 键 词：四逆汤 反渗透 理化参数 浓缩 HPLC特征图谱 

分 类 号：TQ461[化学工程—制药化工]