茵陈蒿汤对二甲基亚硝胺诱导大鼠肝纤维化的逆转作用  

Reverse effect of Yinchenhao decoction in dimethyl nitrosamine-induced hepatic fibrosis in rats

作　　者：王永宏[1] 赵晨曦[1] 陈本美[2] 贺敏[3] 刘琳琪[1] 李春艳[1] 陈新[1] 

机构地区：[1]长沙学院生物工程与环境科学系,湖南长沙410022 [2]中南大学现代分析测试中心,湖南长沙410008 [3]湘潭大学化工学院制药工程系,湖南湘潭411105

出　　处：《中国中药杂志》2014年第8期1473-1478,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81173533);湖南省教育厅科学研究重点项目(11A017);长沙市科技计划重点项目(K110703-11)

摘　　要：目的:探讨茵陈蒿汤抗二甲基亚硝胺(DMN)诱导的大鼠肝纤维化作用。方法:采用1%DMN按照1 mL给予大鼠腹腔注射制备肝纤维化模型,每周前3 d每天1次,连续造模3周;造模当天开始灌胃给药水飞蓟素(阳性对照组,50 mg·kg-1·d-1)、茵陈蒿汤(高、中、低剂量治疗组,20.0,8.0,3.2 g·kg-1·d-1),模型组和正常对照组大鼠灌胃生理盐水,连续干预5周;采用HE染色观察肝组织病理学变化;测定血清中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、γ-谷氨酰转移酶(γ-GGT)、透明质酸(HA)、层粘连蛋白(LN)、Ⅳ型胶原(CⅣ)和Ⅲ型前胶原氨端肽(PⅢNP)水平;测定肝组织氨基酸代谢谱及羟脯氨酸含量。结果:与模型组比较,茵陈蒿汤高、中剂量能显著逆转大鼠肝组织病理学变化;茵陈蒿汤能剂量依赖性降低大鼠血清中ALT,AST,γ-GGT,HA,LN,CⅣ及PⅢNP水平;茵陈蒿汤能剂量依赖性降低肝组织羟脯氨酸含量,并能改变大鼠肝组织中氨基酸代谢谱。结论:茵陈蒿汤具有明显逆转二甲基亚硝胺诱导所致大鼠肝纤维化的作用。Objective： To discuss the reverse effect of Yinchenhao decoction（YCHD） in dimethyl nitrosamine（DMN） -induced hepatic fibrosis in rats. Method： The rat hepatic fibrosis model was established through the intraperitoneal injection with 1% dimethyl nitrosamine （DMN） with a dose of 1.0 mL kg-1 d-1 for consecutively three weeks, once for the first three days of each. The rats were randomly divided into six groups： the silymarin positive control group （50. 0 mg kg-1 d-1 ）, YCHD high （20. 0 g kg-1 d-1 ）, middle （8.0 g kg-1 d-1 ） and low （3.2 g kg-1 d-1 ） dose groups, the model group and the normal control group. The model group and the normal control group were orally administered with normal saline for consecutively five weeks. The pathologic changes in liver tissues were observed by HE staining. The levels of alanine aminotransferase （ ALT ） , aspartate aminotransferase （AST）, T-glutamyhransferase（T-GGT）, hyaluronic acid（HA）, laminin（LN）, collagen type IV（C1V） and type III procollagen ami- no terminal peptide （Pill NP） in serum were determined. The metabolite profiling of amino acid and the content of hydroxyproline in liver tissues were also measured. Result： Compared with the model group, YCHD high and middle dose groups could significantly re- verse the pathologic changes in liver tissues of rats. YCHD could reduce the levels of ALT, AST, T-GGT, HA, LN, CIV, PIIINP in serum and the content of hydroxyproline in liver tissues in a dose-dependent manner, and altered the metabolite profiling of amino acid in rat liver tissues. Conclusion： YCHD has the effect in reversing dimethyl nitrosamine induced hepatic fibrosis in rats.

关 键 词：茵陈蒿汤 肝纤维化 二甲基亚硝胺 羟脯氨酸 

分 类 号：R285.5[医药卫生—中药学]