西黄丸抗肿瘤作用及其免疫清除功能的实验研究  

Experimental study on anti-tumor effect of Xihuang pill and its immune clearance function

作　　者：马杰[1] 王一尧[2] 杨伟[2] 关硕[2] 曾常茜[1] 高文斌[3] 梁文波[1] 

机构地区：[1]遵义医学院,贵州遵义563000 [2]大连大学医学院,辽宁大连116622 [3]大连大学附属中山医院肿瘤科,辽宁大连116001

出　　处：《中国中药杂志》2014年第8期1499-1501,共3页China Journal of Chinese Materia Medica

基　　金：大连市金州新区高新技术研究开发项目(2012-A1-046)

摘　　要：目的:探讨西黄丸对荷瘤大鼠的抗肿瘤作用及其对荷瘤机体免疫清除功能的影响。方法:采用Walker256建立大鼠荷瘤模型,随机分为空白对照组、模型对照组、香菇多糖组、西黄丸低、中、高剂量组,每组10只。造模后连续治疗给药14d。腹主动脉取血,取瘤,计算抑瘤率;采用流式细胞技术(FCM)检测外周血中CD3+,CD4+,CD8+T细胞及黏附分子B7-1(CD80)的含量;ELISA测定外周血IL-2,IFN-γ表达水平。结果:西黄丸高剂量组抑瘤率为33.1%;与对照组比较,模型组大鼠外周血中IL-2,IFN-γ水平及CD3+,CD4+,B7-1含量明显降低,差异有统计学意义(P<0.05);与模型组比较,西黄丸高剂量组大鼠外周血中IL-2,IFN-γ水平及CD3+,CD4+,B7-1含量增高(P<0.05)。结论:西黄丸可通过提高外周血IL-2,IFN-γ水平及CD3+,CD4+,B7-1含量,增强免疫清除功能,发挥其抗肿瘤作用。Objective： To discuss the anti-tumor effect of Xihuang pill on tumor-bearing rats and its effect on the immune clearance function of tumor-beating organisms. Method： Walker256 tumor cells were adopted to establish the tumor-bearing rat model. The rats were randomly divided into five groups： the normal control group, the model control group, the lentinan group and Xihuang pill low dose, middle dose and high dose groups, with 10 rats in each group, and continuously treated and given drugs for 14 d after modeling. Blood and tumors were collected from abdominal aorta to calculate the tumor inhibition rate. The content of CD3＋ , CD4＋ , CD8＋T cells and adhesion molecule B7-1 （CD80） in peripheral blood were detected by flow cytometry （FCM）. The expressions of IL-2 and IFN-γ in were determined by ELISA. Result： The tumor inhibition rate of the Xihuang pill high dose group was 33.1 percent. Compared with the model group, the Xihuang pill large dose group showed significantly low IL-2 ,IFN-γ, CD3＋ , CD4＋ , B7-1 in peripheral blood, with statistical significance in their differences （ P 〈 O. 05 ）. Conclusion ： Xihuang pill could show its anti-tumor effect by enhancing the immune clearance function and increasing IL-2, IFN-γ, CD3＋T, CD4＋T, B7-1 in peripheral blood.

关 键 词：西黄丸 Walker256乳腺癌细胞 荷瘤大鼠 免疫清除 

分 类 号：R285.5[医药卫生—中药学]