子痫前期发病及其严重程度与NF-κB、TNF-α及PLGF相关性研究  

Study on the correlation between onset,degree of severity of preeclampsia and NF- κB,TNF- α,PLGF

作　　者：李新菊[1] 

机构地区：[1]山东省潍坊市人民医院输血科,261041

出　　处：《中国妇幼保健》2014年第12期1849-1851,共3页Maternal and Child Health Care of China

摘　　要：目的:探讨核转录因子(NF-κB)、胎盘生长因子(PLGF)、肿瘤坏死因子(TNF-α)与子痫前期发病的关系,为临床诊断及治疗提供依据。方法:选取60例子痫前期患者(观察组,其中轻度30例,重度30例)和30例正常孕妇(对照组),采用酶联免疫吸附法(ELISA)对观察组和对照组患者的血清TNF-α和PLGF水平以及胎盘中NF-κB、TNF-α和PLGF水平进行检测,对三者的相关性进行分析。结果:观察组患者胎盘组织中TNF-α和NF-κB表达水平显著高于对照组(P<0.05),观察组患者血清PLGF表达水平显著低于对照组(P<0.05)。观察组中重度子痫前期组患者TNF-α和NF-κB表达水平显著低于轻度子痫前期组(P<0.05),二者PLGF表达水平相比无统计学差异(P<0.05)。且NF-κB及TNF-α与PLGF表达强度呈显著负相关,NF-κB与TNF-α表达强度呈显著正相关。观察组患者血清TNF-α水平显著高于对照组(P<0.05),观察组患者血清PLGF的水平显著低于对照组(P<0.05)。观察组中重度子痫前期组患者血清TNF-α水平较轻度子痫前期组显著增高(P<0.05),血清PLGF水平显著低于轻度子痫前期组(P<0.05),且观察组患者血清TNF-α与PLGF水平呈显著负相关。结论:在子痫前期发生过程中,TNF-α、PLGF、NF-κB三种细胞因子间存在一定的联系,可能通过相关机制参与子痫前期发病,并且三类物质与子痫前期的进展及其病情严重程度有密切关系。Objeαive： To explore the relationship between nuclear transcription faαor （NF - KB）, placental growth faαor （PL- GF）, tumor necrosis faαor - α （TNF - α） and onset of preeclampsia, provide a basis for clinical diagnosis and treatment. Methods： Sixty patients with preeclampsia （ observation group, including 30 patients with mild preeclampsia and 30 patients with severe preeclampsia） and 30 normal pregnant women （control group） were seleαed; ELISA was used to deteα the serum levels of TNF -α and PLGF, placental levels of NF - KB, TNF - α and PLGF in the two groups ; their correlation was analyzed. Results ： The expression levels of TNF - α and NF - KB in placental tissue of observation group were statistically significantly higher than those in control group （ P 〈 0. 05 ） , the expression level of PLGF in serum of observation group was statistically significantly lower than that in control group （ P 〈 O. 05 ） . The expression levels ofTNF - α and NF - KB in severe preeclampsia subgroup were statistically significantly lower than those in mild preeclampsia subgroup （ P 〈 0. 05）, but there was no statistically significant difference in the expression level of PLGF between severe preeclampsia subgroup and mild preeclampsia subgroup （ P 〈 O. 05 ） . There was a significantly negative correlation between NF - KB, TNF - α expression levels and PLGF expression level, there was a significantly positive correlation between NF - KB expression level and TNF - α expression level. The expres- sion level of TNF - α in serum of observation group was statistically significantly higher than that in control group （ P 〈 O. 05 ）, the expression level of PLGF in serum of observation group was statistically significantly lower than that in control group （ P 〈 O. 05 ） . The expression level of TNF-α in serum of severe preeclampsia subgroup was statistically significantly higher than that of mild preeclampsia subgroup （P 〈 0.05 ）, the expression level of PLG

关 键 词：子痫前期 核转录因子-KB 肿瘤坏死因子Α 胎盘生长因子 

分 类 号：R714.245[医药卫生—妇产科学]