西藏灵芝多糖的提取工艺研究  

Study on extraction process of polysaccharides from Ganoderma lucidum of Tibet

作　　者：林秀仙[1] 李菁[1] 陈亮[1] 罗爱勤[1] 

机构地区：[1]广州白云山汉方现代药业有限公司中药提取分离过程现代化国家工程研究中心,广州510240

出　　处：《中国当代医药》2014年第11期8-10,13,共4页China Modern Medicine

基　　金：广东省广州市科技计划项目(201300000084)

摘　　要：目的优化西藏灵芝多糖的提取工艺。方法以蒽酮-硫酸法作为灵芝多糖含量的测定方法,以多糖提取得率为考察指标,对浸提过程中的温度、时间、次数以及料液比4个因素进行单因素试验和正交试验。结果灵芝多糖的最佳提取条件为提取温度100℃,浸提时间2 h,浸提次数3次,料液比为1∶30。结论该提取工艺条件稳定,重现性好,可为西藏灵芝多糖的大生产提供参考依据。Objective To optimize the extraction process for polysaccharides from Ganoderma lucidum of Tibet. Meth- ods Anthrone-sulphuric acid method was used to dectect the content of polysaccharides,extraction rate of polysaccha- rides was took as inspection indicator,four factors such as temperature,time,number of times and ratio of material to liq- uid in the leaching process was conducted single-factor test and orthogonal test. Results The optimum temperature of extracting was 100℃,the optimum times of extracting was 2 times,the optimum extracting time was 2 h and ratio of ma- terial to liquid was 1：30 of Ganoderma lucidum polysaccharides. Conclusion The condition of extracting process are stable and it is reproducible,which can provide reference for preparation of polysaccharides from Ganoderma lucidum of Tibet.

关 键 词：西藏灵芝 多糖 提取工艺 含量测定 

分 类 号：R931.5[医药卫生—生药学]