过氧化聚吡咯膜修饰电极色谱电化学用于帕金森试验动物的研究  

Study of Experimental Parkinsonian Animal by Using High Performance Liquid Chromatography with Polymer-Overoxidized Polypyrrole Film Chemically Modified Electrode

作　　者：张文[1] 许群[1] 曹旭妮[1] 刘梅川[1] 金利通[1] 

机构地区：[1]华东师范大学化学与生命科学学院,上海200062

出　　处：《分析化学》2001年第2期133-137,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金!(29975009)资助项目。

摘　　要：研制了一种新型的过氧化聚吡咯膜修饰电极（ＯＰＰｙ／ＣＭＥ）作为液相色谱电化学检测器，该电极可以用来检测生物体内的单胺类神经递质及其代谢产物。用药物建立了帕金森动物试验模型，对不同情况下的试验动物脑内单胺类神经递质及其代谢产物通过微渗析取样和液相色谱电化学检测联用技术进行了测定。初步探讨了试验动物产生帕金森病的机理，为研制和筛选更有效的治疗帕金森病的新药提供了一种准确、可靠的分析手段。A novel polymer - overoxidized polypyrrole film chemically modified electrode( CME) as the liquid chromatography-electrochemical detector (LC-ECD) was fabricated and applicated, which could be used to determine the monoamine neurotransmitters. The experimental model of parkinsonian animal was established by using medicine. Microdialysis sampling coupled with LC-ECD was used to monitor the monoamine neurotransmitters end their metabolites in experimental animal brain in vivo under different situations. The mechanism of experimental animal's parkinsonian disease was studied elementarily. An accurate and reliable analysis method was supplied for filting the new and the more effective medicine to cure parkinsonian disease.

关 键 词：过氧化聚吡咯膜修饰电极 色谱电化学 帕金森试验动物 帕金森病 单胺类神经递质 代谢产物 测定 

分 类 号：O646[理学—物理化学]