金属离子存在下5-氟尿嘧啶与脱氧核糖核酸、血清白蛋白的相互作用  

The Interaction of 5-Fluorouracil with Deoxyribonucleic Acid and Serum Albumin

作　　者：卢继新[1] 张贵珠[2] 王月梅[2] 

机构地区：[1]天津医科大学药学院,天津300203 [2]南开大学化学系,天津300071

出　　处：《分析化学》2001年第2期192-194,共3页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金!(No.29575200);天津医科大学自然科学基金资助课题。

摘　　要：用紫外光谱、荧光光谱、圆二色谱法等方法研究了抗癌药物５－氟尿嘧啶（５－ｆｌｕｏｒｏｕｒａｃｉｌ，５－ＦＵ）与ＤＮＡ、血清蛋白的相互作用及某些二价金属离子对其作用的影响。求得了５－ＦＵ与ＨＳＡ、ＢＳＡ的结合常数。The interaction of 5-fluorouracil (5-FU) with deoxyribonucleic acid (DNA) and serum albumin and the influence of some metal ions on the interaction have been studied by using spectral methods of UV-absorption, fluorescence and circular dichroism. It was shown that the interaction of 5-FU with DNA and serum albumin was effected by some metal ions. The binding costant of 5-FU with serum albumin was found to be K-HSA = 4.38 x 10(3) (mol/L)(-1) and K-BSA = 4.95 x 10(3) (mol/L)(-1) and the distance between the bound 5-FU and the tryptophan residue mrs also determined to be r(HSA) = 3.53 nm, r(BSA) = 3.51 nm.

关 键 词：5-氟尿嘧啶 脱氧核糖核酸 血清白蛋白 金属离子 抗肿瘤药物 药理作用 药物代谢 

分 类 号：O629[理学—有机化学]