高效液相色谱-脉冲安培检测法测定脑微透析溶液中单胺类神经递质  

Determination of Monoamine Neurotransmitters and Related Compounds in Microdialysis Solution by High Performace Liquid Chromatography with Pulsed Amperometric Detection

作　　者：徐桂芸[1] 常溪燕[2] 常理文[1] 

机构地区：[1]中国科学院化学研究所,分子科学中心北京100080 [2]北京石油化工科学研究院

出　　处：《分析化学》2001年第2期199-201,共3页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助项目!(No.29775027)

摘　　要：详细对比了脉冲安培与恒电位安培检测单胺类神经递质的响应值，结果表明脉冲安培检测上述物质的响应值是恒电位安培检测的１．２倍。建立了以３．４二羟基苄胺（ＤＨＢＡ）为内标的定量方法。在微透析溶液中的微量神经递质的回收率在７４％～１０１％之间。A method for determination of monoamine neurotransmitters and related compounds in microdialysis solution by high performance liquid chromatography (HPLC) with pulsed amperometric detection was described. A comparison of response of catecholamines between iso potential amperometric (AD) and pulsed amperometric detection(PAD) was made, The results indicated that the sensitivity of PAD was 1.2 fold that of AD. The quantitative determination of monoamine neurotransmitters and related compounds was carried out with 3,4-dihydroxybenzylamine (DHBA) as internal standard. The recoveries of monoamine neurotransmitters in microdialysis solution are 71% similar to 101%.

关 键 词：单胺类神经递质 脉冲安培检测 高效液相色谱 脑微透析液 测定 

分 类 号：O657.7[理学—分析化学]