Effects of Cultivation Conditions on Chlorophyll Biosynthesis and Carotenoid Accumulation of the Microalga Chlorella Fusca  

作　　者：Kazuo Kondo Shin Okamoto Michiaki Matsumoto 

机构地区：[1]Department of Chemical Engineering and Materials Science, Doshisha University, Kyoto 610-0321, Japan

出　　处：《Journal of Chemistry and Chemical Engineering》2013年第5期431-440,共10页化学与化工（英文版）

摘　　要：The purpose of the present study is to optimize the cultivation conditions for the productivities of chlorophyll and carotenoid of the microalga chlorellafusca in a flow bioreactor. The effects of CO2 concentration in aeration and light intensity as the culture condition were firstly examined. After the cultivation conditions as to CO2 concentration and light intensity were determined, the effects of the concentrations of KNO3, CH3COONa, NaC1 and MgSO4 in the culture medium on the chlorophyll biosynthesis and carotenoid accumulation ofC. fusca were examined. KNO3 and CH3COONa were used as the nitrogen and the organic carbon sources, respectively. They are very important nutrient sources to the microalgae. In fact, when the concentration of the nutrient sources was increased, the syntheses of chlorophyll and the carotenoid accumulation of the microalgae were promoted. Cfusca can take an organic carbon as the nutrient sources for its own growth, so the cell is activated compared to the case only in an inorganic nourishment sources. Mg2＋ is known as a raw material of chlorophyll and it can promote carotenoid accumulation. However, when it exists in excessive quantity, the microalgae receive a stress and it was difficult for microalgae to uptake such nutrient sources. In this study, it was found that there existed the optimum amounts of KNO3, CH3COONa, NaCI and MgSO4 which should be added to the culture medium.

关 键 词：Chlorophyll CAROTENOID MICROALGAE chlorella fusca tubular bioreactor photoautotrophic cultivation. 

分 类 号：S968.4[农业科学—水产养殖] Q945.11[农业科学—水产科学]