TIPE2在类风湿性关节炎患者中的表达及其对NF-κB信号通路的影响  

The expression of TIPE2 in patients with rheumatoid arthritis and its effect to NF-κB pathway

作　　者：石改绍[1] 武新峰[1] 王飞[1] 史晓飞[1] 

机构地区：[1]河南科技大学第一附属医院风湿免疫科,河南洛阳471003

出　　处：《中国现代医学杂志》2014年第4期34-37,共4页China Journal of Modern Medicine

摘　　要：目的检测类风湿性关节炎患者中TIPE2表达的变化,并探讨其相关的临床意义。方法收集2010年10月~2012年12月该院风湿免疫科收治的类风湿关节炎病例88例,分为中高活动期50例,低活动38例,另取该院体检中心健康体检者80例作为正常对照组。TIPE2 mRNA表达使用荧光定量PCR检测,C反应蛋白（CRP）使用乳胶增强免疫比浊法检测,抗链球菌O（ASO）使用快速乳胶凝集试验检测,类风湿因子（RF）和P65使用ELISA法。结果对照组和类风湿关节炎患者NF-κB信号通路中P65蛋白的表达分别为（1.54±0.41）和（3.44±1.02）μg/mL,各组之间差异具有显著性（P〈0.05）,类风湿关节炎患者外周血单核细胞TIPE2mRNA的表达水平为（0.24±0.08）显著低于对照组的（0.43±0.12）（P〈0.05）。类风湿关节炎高活动期和低活动期患者在年龄、性别等临床资料方面差异无显著性（P〉0.05）,中高活动期患者DAS评分显著高于低活动患者（P〈0.01）。非急性期患者TIPE2表达水平为（0.34±0.11）显著高于急性期患者的（0.16±0.06）（P〈0.05）,而CRP、RF、ASO的低活动组均显著低于中高活动期组（P〈0.01）。结论 TIPE2的低表达参与了类风湿关节炎病变的发生、发展,TIPE2表达下调激活了NF-κB信号通路可能是其致病的主要机制。【Objective】To investigate the expression of TIPE2 in patients with rheumatoid arthritis（RA） and its clinical significance.【Methods】A total of 88 cases of RA patients were enrolled in this study. RA patients were divided into two groups： medium and high activity group（n =50） and low activity group（n =38）. Another 80 healthy person were used as a normal control. The mRNA levels of TIPE2 weremeasured by Real-time PCR analysis. The levels of serum C-reactive protein（CRP） were measured by Latex enhanced immunoturbidimetric analysis. Rapid latex agglutination test was used to detect anti-streptococcus O（ASO） expression. The serum rheumatoid factor（RF） and P65 were measured by ELISA analysis.【Results】The P65 protein of NF-κB signaling pathway was（1.54±0.41） and（3.44±1.02） μg/mL in control group and RA patients group. There was significant difference between the two groups（P 0.05）. The mRNA expression of TIPE2 in AF patients was（0.24 ± 0.08）, and which was significantly lower than（0.43 ± 0.12） of the control group（P 0.05）. There was no significant difference of age and gender between the medium and high activity group and low activity group（P 0.05）. The DAS scores were higher in medium and high activity group than that in low activity group（P 0.01）. The mRNA expression of TIPE2 in low activity group was（0.34±0.11）, and which was significantly higher than that of（0.16±0.06） in medium and high activity（P 0.05）. The expression of CRP, RF, and ASO in low activity group was lower than that of in medium and high activity（P 0.05）. 【Conclusion】The low-level expression of TIPE2 involved in occurrence and development of rheumatoid arthritis disease. NF-κB signaling pathway was activated by downregulated TIPE2, which may be the main mechanism of pathogenicity.

关 键 词：类风湿关节炎 TIPE2 NF-ΚB信号通路 

分 类 号：R593.22[医药卫生—内科学]