中国产Ganoderma lucidum的系统发育学分析及栽培技术研究(英文)  

Phylogenetic Analysis and Cultivation Technique of Ganoderma lucidumfrom China

作　　者：陈永敢[1] 陈光宙[1] 袁学军[1] 陈忠荫[1] 陈川平[1] 张来军[1] 

机构地区：[1]琼州学院生物科学与技术学院,海南三亚572022

出　　处：《北方园艺》2014年第8期92-96,共5页Northern Horticulture

基　　金：三亚市院地科技合作资助项目(2012YD09,2011YD06);琼州学院青年教师科研基金资助项目(QYQN201235)

摘　　要：灵芝是一类木腐真菌,广泛存在于热带雨林中。根据形态特征、rDNA-ITS和mt SSU的系统发育学分析,课题组将从Cyclobalanopsis bambusaefolia分离出来的菌株命名为G.lucidum,该菌株可人工培养。系统发育学分析表明,这些菌株类群与亚洲灵芝为一个独立的分支;此外,该研究采用传统椴木栽培技术,实现了分离菌株的人工栽培。人工栽培灵芝子实体粗厚,木栓质,边缘淡黄,在成熟的过程中逐渐变褐。Ganoderma strains are wood-rotting fungi and popular living in tropical forest. Based on the morphological characteristics, phylogenetics analysis of rDNA-ITS and mitochondrial small- subunit ribosomal DNA sequences, we proposed isolates collected from Cyclobalanopsis bambusaefolia grown in China were G. lucidum, also this group isolates could be artificial cultivation. Morphology,host distribution and molecular phylogenetic evidence supported recognition of this species. Phylogenetic analysis indicated these isolates clustered with Asian G. lucidum into a distinct clade. Artificial cultivation showed fruiting bodies were thick, corky, and yellowish in the growing margins and then turn to brownish in the matured part on the surface.

关 键 词：系统发育学 RDNA-ITS mt SSU GANODERMA lucidum 

分 类 号：S567.31[农业科学—中草药栽培]