复方中药禽康散对接种新城疫病毒鸡胚的保护效应  

The protective effect of compound Chinese herbal medicine Qinkangsan on the chick embryos inoculated with Newcastle disease virus

作　　者：邓惠丹[1] 曾加齐 邓俊良[1] 申渝波[1,3] 杨军[1,4] 左之才[1] 任志华[1] 王娅[1] 

机构地区：[1]四川农业大学动物医学院/动物疫病与人类健康四川省重点实验室/环境公害与动物疾病四川省高校重点实验室,四川雅安625014 [2]眉山市丹棱县杨场镇畜牧兽医站,四川眉山620200 [3]通威集团药物研究所,成都620000 [4]贵州省毕节市农牧局,贵州毕节551700

出　　处：《黑龙江畜牧兽医（下半月）》2014年第4期82-83,88,共3页

基　　金：教育部"长江学者和创新团队发展计划"创新团队项目(IRT0848);四川省教育厅自然科学重点项目(2006A009);四川省科技厅应用基础研究项目(2008JY0098)

摘　　要：为了研究复方中药禽康散提取液对鸡新城疫病毒活性的影响,试验采用鸡胚法以不同浓度中药提取液与新城疫F48E9毒株悬液混合后各接种8枚鸡胚,观察各组鸡胚的存活情况。结果表明:中药浓度为1.0 g/mL、0.5 g/mL、0.25 g/mL时活胚率分别是87.5%、75.0%、50.0%,而不加中药组鸡胚96 h后全部死亡。说明复方中药禽康散具有抗新城疫F48E9毒株的作用,且随着中药浓度的增加抗病毒作用增强,呈现一定的量效关系。To study the effect of the extracting solution from compound Chinese herbal medicine Qinkangsan on the activity of Newcastle disease virus （NDV）, eight chick embryos in each group were inoculated with different concentrations of the extracting solution from Qinkangsan and mixed with the suspension of NDV strain F48E9, respectively, using the method of chick - embryo culture. The survival rates of the chick embryos in each group were observed. The results showed that the survival rates of chick embryos with different concentrations of Qinkangsan （ 1. 0 g/mE, 0.5 g/mL, and 0.25 g/mL, respectively） were 87.5% , 75.0% , and 50.0% , respectively. All the chick embryos in the group without Qinkangsan died after 96 h. The results indicate that compound Chinese herbal medicine Qinkangsan has an effect against NDV strain F48E9, and the effect is increased with increasing concentrations of Qinkangsan, showing a certain dose -effect relationship.

关 键 词：中药 禽康散 新城疫病毒 鸡胚 活性 

分 类 号：S858.31[农业科学—临床兽医学] R282[农业科学—兽医学]