测定葡萄糖的简易分光光度法  

Simple Method for Determination of Glucose by Spectrophotometry

作　　者：王长发[1] 熊松[1] 向阳[1] 吴志强[1] 蒋勉[1] 

机构地区：[1]武汉大学化学系,武汉430072

出　　处：《分析试验室》2001年第1期17-18,共2页Chinese Journal of Analysis Laboratory

基　　金：教育部跨世纪人才基金

摘　　要：研究发现葡萄糖与磷钼杂多酸反应 ,能生成有色产物 ,它的最大吸收波长是 690 nm,吸光度与葡萄糖的质量浓度呈线性关系 ,检出限为 2× 1 0 -5mol/L。本法已用于测定葡萄糖注射液中的葡萄糖含量。A spectrophotometric method for determination of glucose was developed based on measurement of color product formed by reaction of glucose with 12 molybdophosphate. The absorbance is proportional to concentration of glucose over the range of 2×10 -5 ～2×10 -3 mol/L with detection limit of 2×10 -5 mol/L at wavelength of 690 nm.The method was applied to determination of glucose in injectors with satisfactory results.

关 键 词：葡萄糖 磷钼杂多酸 分光光度法 测定 葡萄糖注射液 

分 类 号：R977.6[医药卫生—药品] R927.2[医药卫生—药学]