化学诱变枯草芽孢杆菌对大白菜根肿病的防治效果  

Control Effect of Chemically Induced Bacillus subtilis on Chinese Cabbage Clubroot Disease

作　　者：杨晶晶[1] 陈丽华[1] 李兴玉[1] 吴毅歆[2] 陈映岚 何月秋[1,2] 

机构地区：[1]云南农业大学农业生物多样性应用技术国家工程中心,云南昆明650201 [2]云南农业大学农学与生物技术学院,云南昆明650201 [3]云南省禄劝县农业技术推广总站,云南禄劝651500

出　　处：《安徽农业科学》2014年第9期2597-2599,共3页Journal of Anhui Agricultural Sciences

基　　金：农业部公益性行业(农业)科研专项(201003029);云南省支撑计划项目(2009EB060)

摘　　要：[目的]研究化学诱变枯草芽孢杆菌对大白菜根肿病的防治效果。[方法]采用3株来自枯草芽孢杆菌XF-1的化学诱变菌株XF-1A、XF-1C和XF-1E进行大白菜根肿病的盆栽防病试验,并以MALDI-TOF-MS分析了3种抗生素的相对含量。[结果]菌株XF-1A和XF-1C防效较好,分别为68.80%和65.06%,XF-1E防效较差为42.21%。3菌株均能分泌表面活性素、伊枯草菌素和丰源素,但表面活性素和伊枯草菌素相对含量为XF-1A>XF-1C>XF-1E,XF-1A的表面活性素和伊枯草菌素分别达29.26%和35.31%,XF-1E的丰源素含量最高,达41.09%。[ Objective] The aim was to study control effect of chemically induced Bacillus subtilis on Chinese cabbage clubroot disease. [ Method] Three mutants, XF-1 A, XF-1C and XF-1E chemically induced from a wild strain, Bacillus subtilis XF-1, were used to control clubroot disease of Chinese cabbage in pot experiment and to analyze their antibiotics by MALDI-TOF-MS technique. [ Result ] The results showed that XF-1A and XF-1C had 68.80% and 65.06% control effect, respectively, and XF-1E had 42.20% control effect. These strains could produce surfactin, iturin and fengycin. But they produced the relative amount of iturin and surfactin in order of XF-1A 〉 XF-1C 〉 XF- 1E. XF-1A contained 29.26% and 35.31% of surfactin and iturin, XF-1E had highest amount of 41.09% fengycin. [ Conclusion] Surfactin and iturin may be associated with the disease control effect.

关 键 词：根肿病 表面活性素 伊枯草菌素 丰源素 

分 类 号：S432.42[农业科学—植物病理学]