多杀菌素高产菌株的诱变选育及代谢曲线初步研究  

Screening and breeding of high spinosad producing strains and preliminary study on metabolism curve of mutation

作　　者：郭伟群[1] 邹球龙[1] 陈园[1] 李能威[1] 张晓琳[1] 

机构地区：[1]国家粮食局科学研究院,北京100037

出　　处：《中国抗生素杂志》2014年第4期245-248,共4页Chinese Journal of Antibiotics

基　　金："十二五"科技支撑计划项目子课题"储粮害虫生物防护剂及真菌真菌毒素降解剂的研发与示范"(课题编号:2011BAD03B02-2)

摘　　要：目的研究60Co-γ射线对刺糖多孢菌的诱变效应,期望获得性状稳定的多杀菌素高产菌株。方法以N-19为出发菌株,通过60Co-γ射线诱变,利用摇瓶发酵结合HPLC法测定进行筛选,通过摇瓶定期取样方法研究了突变株的代谢曲线。结果辐照剂量90Gy、120Gy刺糖多孢菌的正突变率较高;通过5轮复筛及传代验证最终获得4株产量提高、性状稳定的突变株;高产突变株在摇瓶中延滞期较短且产素与生物量的增长成正相关。结论利用60Co-γ射线辐照诱变选育是获得多杀菌素高产菌株的有效手段。Objective 60Co-γ irradiation was employed to generate mutations in Saccharopolyspora spinosa in order to obtain stable spinosad over-production strain. Methods 60Co-γ irradiation was used to mutate S. spinosa N-19 strain. The mutants were screened by shaking flask and HPLC. Metabolism curve of the mutant were studied by Periodic sampling. Results The positive mutants with larger proportion were obtained when the spores of the original strain were treated by 90Gy and 120Gy of y-ray for 50min. Three mutants were obtained through five rounds screening and they showed enhanced spinosad production by 34.49%, 25.09% and 23.33% compared to original strain. The flask culture process of the high yield mutants was analysed. The lag phase of growth is short, the high yields and biomass are directly related. Conclusion 60Co-γ irradiation is an efficient method for obtaining stable spinosad over- production strain.

关 键 词：刺糖多孢菌 多杀菌素 60Co-γ 射线诱变 代谢曲线 

分 类 号：R978.1[医药卫生—药品]