星点设计-效应面法优化去甲斑蝥素-齐墩果酸复合脂质体的制备工艺  

Optimization of preparation process of norcantharidin and oleanolic acid liposome by Central composite design-response surface method

作　　者：刘道刚[1] 方志娥[1] 唐直婕[2] 邹亮[3] 冷静[1] 

机构地区：[1]重庆市中医院,重庆400021 [2]重庆医科大学药学院,重庆400016 [3]成都大学生物产业学院,四川成都610106

出　　处：《华西药学杂志》2014年第2期125-128,共4页West China Journal of Pharmaceutical Sciences

基　　金：重庆市自然科学基金计划项目(CSTC;2010BB5347);重庆市卫生局中医药科技项目(2011-2-161);四川省中医药管理局青年基金项目(200802)

摘　　要：目的制备去甲斑蝥素-齐墩果酸复合脂质体(NCTD-OA-LP)。方法以薄膜分散法制备脂质体,采用星点设计,以去甲斑蝥素和齐墩果酸的包封率为指标,对两种指标进行归一化处理,采用效应面法优化脂质体的处方工艺。结果筛选的最优处方为药物和磷脂、胆固醇和磷脂的重量百分比分别为11.8%、17.2%,超声20 min。复合脂质体中,去甲斑蝥素和齐墩果酸的包封率分别为45.6%、84.5%。结论经星点设计-效应面法优化后制得的NCTD-OA-LP具有较好的包封率。OBJECTIVE To prepare norcantharidin and oleanolic liposome （ NCTD - OA - LP）. METHODS To prepare lipo- some by film hydration method, Central composite design - response surface method and overall desirability were used to optimize the formulation with the encapsulation efficiency of NCTD and OA as indicator. RESULTS The optimum conditions of formulation were NCTD and OA/phosphatidylcholine of 11.8% , cholesterol/phosphatidylcholine of 17.2% , and ultrasonic time was 20 min. The encap- sulation efficiency of NCTD and OA were 45.6% and 84.5% , respectively. CONCLUSION The NCTD - OA - LP shows good encapsulation efficiency after optimized with Central composite design -response surface method.

关 键 词：去甲斑蝥素 齐墩果酸 脂质体 制备工艺 星点设计-效应面法 

分 类 号：R94[医药卫生—药剂学]