丙戊酸钠对SCN3A基因表达的抑制作用  

Sodium valproate inhibit the expression of SCN3A gene

作　　者：李海军[1,2] 孙筱放[1,2] 

机构地区：[1]广州医科大学附属第三医院 [2]广州市妇产科研究所,广东省产科重大疾病重点实验室,510150

出　　处：《实用医学杂志》2014年第7期1056-1059,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金项目(编号:31171229);广州市科信局项目(编号:2011y1-00038-1)

摘　　要：目的:研究丙戊酸钠在Nav1.3通道基因表达中的作用,为癫痫的临床治疗提供新的依据。方法:通过用丙戊酸钠对N1E-115细胞进行处理,检测丙戊酸钠处理后N1E-115细胞的SCN3A mRNA水平;把SCN3A基因启动子构建成表达载体,转染到N1E-115细胞中,再用丙戊酸钠对转染质粒的细胞进行处理,检测SCN3A mRNA的水平及萤火虫荧光素酶的表达水平。结果:丙戊酸钠对SCN3A具有明显的抑制作用,丙戊酸钠能抑制SCN3A的表达,降低SCN3A蛋白的表达水平。结论:丙戊酸钠通过降低SCN3A的表达达到抑制癫痫发作的作用,开启了对丙戊酸钠治疗癫痫机制的新认识。Objective To determine the role of valproate in the expression of SCN3A and provide new evidence for the clinical treatment of epilepsy. Methods We treated the N1E-115 cells with sodium valproate and examine the expression of SCN3A mRNA. We also constructed the expression vector of the SCN3A gene promoter then transfected the vector into N1E-115 cells,respectively detected the level of SCN3A mRNA and the expression level of firefly luciferase of N1E-115 cells with valproate treated and untreated. Results Valproate inhibited the expression of SCN3A significantly including the level of SCN3A mRNA and luciferase protein. Conclusion Valproate inhibits the seizures by reducing the expression of the SCN3A , which provides a new understanding of the treatment of epilepsy.

关 键 词：癫痫 丙戊酸钠 SCN3A 

分 类 号：R96[医药卫生—药理学]