人参皂甙单体Rg3诱导P27表达变化对人红白血病细胞株K562细胞增殖的影响  

Effects of ginsenoside Rg3-induced P27 expression on proliferation of human erythroleukemia K562 cells

作　　者：马琳娜 梁宇[2] 陈然峰 

机构地区：[1]浙江省武警总队杭州医院检验科,310051 [2]浙江大学医学院附属妇产科医院检验科,杭州市310006

出　　处：《实用医学杂志》2014年第7期1066-1069,共4页The Journal of Practical Medicine

摘　　要：目的:探讨人参皂甙单体Rg3诱导P27表达变化对人红白血病细胞株K562细胞增殖的影响。方法:将人红白血病细胞株K562细胞传代培养至对数生长期,分组加入不同浓度(6.25、12.5、25、50、100μg/mL)的人参皂甙单体Rg3,并设置空白对照组(0μg/mL Rg3)。培养3 d后,瑞氏-姬姆萨染色后,显微镜观察K562细胞生长情况,四氮唑蓝(MTT)比色法测定K562细胞增殖活性,荧光定量RT-PCR法检测P27mRNA的表达。结果 :不同浓度(0、6.25、12.5、25、50、100μg/mL)人参皂甙单体Rg3作用于K562细胞后,MTT比色法测定显示,Rg3组增殖抑制率逐渐升高,与空白对照组相比具有明显差异(P<0.05);荧光定量RTPCR检测结果显示较高浓度(25、50、100μg/mL)人参皂甙Rg3组P27 mRNA表达水平比空白对照组明显升高,差异有显著性(P<0.05)。结论:人参皂甙单体Rg3能通过诱导P27表达升高抑制K562细胞的增殖。Objective To explore the effects of ginsenoside Rg3 on the expression of P27 in human erythrol- eukemia cell line K562 and cell proliferation. Methods Human erythroleukemia cell line K562 cells were cultured to exponential phase, then K562 cells were treated with different concentrations of Rg3 （6.25, 12.5, 25, 50, and 100 μg/mL） as Rg3 group, and cells treated without Rg3 （0 μg/mL） were take as control group. After 3 days, K562 cells were observed by Wright-Giemsa staining with microscopy, the proliferation of K562 cells were examined by tetrazolium salt （MTF） assay, and the expression of P27 mRNA were detected by fluorescent quantitative RT- PCR assay. Results MTT assay showed that after treatment with Rg3, the inhibition rate （IR） of proliferation of cells in Rg3 groups were increased gradually, and the differences were significant compared with the control group （P 〈 0.05）. The resuhs of fluorescent quantitative RT-PCR showed the levels of P27 mRNA expression in 25,50 and 100 μg/mL Rg3 groups were significant higher than that of control group （P 〈 0.05）. Conclusion The ginsenoside Rg3 can inhibit the proliferation of K562 cells by inducing the expression of P27.

关 键 词：人参皂甙RG3 K562细胞 P27 增殖 

分 类 号：R285.5[医药卫生—中药学]