存储时间对血浆中巨细胞病毒DNA稳定性的影响  

Comparative evaluation of cytomegalovirus DNA stability in EDTA plasma over a 21 day period

作　　者：谢丽[1] 易珍[1] 王建[1] 邓燕[1] 陈志坚[1] 徐涓娟[1] 李山[1] 秦雪[1] 

机构地区：[1]广西医科大学第一附属医院检验科,南宁市530021

出　　处：《实用医学杂志》2014年第7期1151-1153,共3页The Journal of Practical Medicine

基　　金：广西壮族自治区卫生厅资助项目(编号:2013034)

摘　　要：目的:了解4℃条件下储存21 d后血浆中巨细胞病毒(cytomegalovirus,CMV)DNA的稳定性。方法:收集CMV载量300～100 000拷贝/mL的30份造血干细胞移植后患者血浆,并分为低载量组、中载量组、高载量组。标本采集后立即分离血浆并进行基线(0 d)载量检测,其余血浆于4℃存储,分别在第1、2、3、7、14和21天进行实时荧光定量PCR测定。结果 :将30份样本进行不同时间点的配对t检验,CMV载量均差异无显著性:第1天与第0天比较,t=1.654,P=0.109;第2天与第0天比较,t=1.487,P=0.148;第3天与第0天比较,t=1.609,P=0.118;第7天与第0天比较,t=0.831,P=0.413;第14天与第0天比较,t=1.721,P=0.096;第21天与第0天比较,t=0.244,P=0.244。分组比较中,第21天与第0天比较CMV载量差异无显著性(低载量组P=0.485;中载量组P=0.858;高载量组P=0.949)。结论:4℃储存21 d中,血浆标本CMV载量未出现连续下降或上升趋势,且不同时间点与第0天的基线载量比较差异无显著性。Objective To analyze CMV DNA stability of 30 EDTA plasma samples in the order of magnitude between 300 and 100 000 eopies/mL over a 21 day period. Methods Thirty plasma samples were grouped into three categories according to the CMV DNA loads, including low CMV DNA contents, intermediate CMV DNA loads and high CMV DNA loads. Ten milliliters of whole blood was freshly collected from each patient. Plasma samples without hemolysis were divided into 1-ml aliquots. One aliquot was processed immediately （Day 0） for baseline PCR assays. The remaining aliquots were then processed after one, two, three, seven, 14 or 21 day of storage at 4 ℃ Results There was no significant difference between the mean of the difference time point in viral loads following storage at 4 ℃ by paired-samples t test, including Day 1 compared to Day 0 （t = 1.654, P = 0.109）, Day 2 compared to Day 0 （t = 1.487, P= 0.148）, Day 3 compared to Day 0 （t = 1.609, P= 0.118）, Day 7 compared to Day 0 （t = 0.831, P = 0.413）, Day 14 compared to Day 0 （t = 1.721, P = 0.096）, and Day 21 compared to Day 0 （t=0.244, P=0.810）. Conclusion The concentration of CMV DNA in all samples stored at 4℃ for 21 days did not differ significantly from the baseline viral load,and it was not observed the trend in continued degradation in different time point （Day 1, 2, 3, 7 and 14）.

关 键 词：存储时间 巨细胞病毒 DNA 稳定性 

分 类 号：R446.5[医药卫生—诊断学]