一种光温敏核不育系提纯繁殖技术的研究  

Research on a Method for Purification and Multiplication of PTGMS Lines

作　　者：何强[1] 庞镇宇[2] 邢俊杰[1] 舒服[1] 张武汉[1] 杨益善[1] 邓华凤[1] 

机构地区：[1]杂交水稻国家重点实验室/湖南杂交水稻研究中心,湖南长沙410125 [2]湖南农业大学水稻研究所,湖南长沙410128

出　　处：《安徽农业科学》2013年第34期13142-13144,共3页Journal of Anhui Agricultural Sciences

基　　金：国家863计划项目(2010AA101305;2011AA10A101)

摘　　要：[目的]研究一种光温敏核不育系提纯繁殖技术。[方法]利用生产用种安农810S、P88S和C815S 3个光温敏核不育系,第1年通过分蔸建立2个遗传背景完全相同的不育系大群体,实现核心单株筛选与繁殖同步进行。所得核心种子于当年在海南严格隔离条件下冬繁获得原原种,并从中筛选优良单株,作为下一年2个遗传背景完全相同供试群体用种。第2年在长沙正季种植所得用种,继续核心单株筛选与繁殖同步进行。所得核心种子又在海南冬繁获得原原种,以后每年重复上述步骤。[结果]安农810S、P88S和C815S群体中核心单株的比例由第1年的40.3%、0.7%和0.3%分别提升到第2年的71.67%、66.00%和68.67%。[结论]该方法经过连续多年的不育系提纯繁殖,能有效控制不育系起点温度"遗传漂移",同时能持续得到高纯度,不育起点温度能得到有效控制的核心种子。[ Objective ] The aims was to explore PTGMS line purification and multiplication technology. [ Method ] Three commercial PTGMS lines, Annong 810S, P88S and C815S, were used in this study. In the first year, two sterile line populations with same genetic background were multiplied by dividing seedling; meanwhile, selection and multiplication of the core individuals were conducted; within the year, the core seeds harvested were multiplied under the isolated condition in Hainan, and at the same time, superior individuals were selected and used as the seeds for two populations with same genetic background in the next year. In the second year, seeds harvested in Hainan were planted in Changsha, while the core individuals were selected and multiplied; the core seeds harvested were continued to multiply in Hainan. The above procedures were repeated in the following years. [ Result] The test results showed that in two years the proportion of core individuals in the population of Annong 810S, P88S and C815S increased from 40.3% , 0.7% and 0.3% in the first year to 71.67% , 66.00% and 68.67% in the second year respectively. [ Conclusion] This suggested that this method of purification and multiplication of the PTGMS lines for consec- utive years can effectively control the drift of the critical sterility inducing temperature, and at the same time, core seeds with high purity and controlled inducing temperature can be harvested.

关 键 词：光温敏核不育系 分蔸 提纯 繁殖 

分 类 号：S517[农业科学—作物学]