蓖麻WRKY39基因的鉴别与表达特性分析  

In Silico Identification and Expression Analysis of WRKY39 Gene from Castor Bean( Ricinus communis L.)

作　　者：邹智[1] 

机构地区：[1]中国热带农业科学院橡胶研究所/农业部橡胶树生物学与遗传资源利用重点实验室,海南儋州571737

出　　处：《安徽农业科学》2013年第34期13311-13315,共5页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学资金(31371556);海南省自然科学基金(312026);中国热带农业科学院橡胶研究所基本科研业务费专项(1630022011014)资助

摘　　要：研究基于已公布的EST数据,采用电子克隆的方法获得了蓖麻WRKY39的基因序列。序列分析表明,该基因包含2个内含子,在释放的基因组中因一碱基G的缺失而被错误注释成2个基因;基因预测编码356个氨基酸,理论分子量为38.95 kD,等电点为9.43;蛋白含有一个保守的WRKY结构域,其基序为WRKYGQK_X13_C_X5_C_X23_H_X1_H,归属于II类d亚类,其保守内含子位于304位的R和305位的G之间;基因的启动子区含有多种激素和胁迫应答相关元件,显示出复杂的转录调控网络及在胁迫应答中的重要功能。基因表达谱分析显示,RcWRKY39在叶片、花、胚乳和种子中都有表达,其中在种子中的表达丰度最高,在叶片和花中次之,在胚乳中最低。WRKY is a plant-specific transcription factor family involved in plant response to biotic and abiotic stresses. In this study, a WRKY transcription factor denoted as RcWRKY39 was in silico cloned based on the released EST sequences. Sequence analysis showed that RcWRKY39 contains two introns, and was misannoted as two genes which was caused by missing a base G during sequencing; the gene en- codes a deduced peptide of 356 amino acids with theoretical molecular weight of 38.95 kDa and isoelectric point of 9.43 ; the protein contains one WRKY domain with a pattern of WRKYGQK X13_C_XS_C X23_H_Xl_H, and can be grouped into class II subclass d with the conser- vative intron located between site R304 and site G305. A variety of hormone and stress-responsive cis-acting elements found in the promoter region suggest a complex transcription regulatory network and its potential role in stress response. Digital expression profiling showed that Rc- WRKY39 expressed in castor bean leaves, flowers, endosperms and seeds, with the highest expression level in seeds, then, lower in leaves and flowers, and lowest in the endosperms.

关 键 词：蓖麻 WRKY转录因子 电子克隆 生物信息学分析 RICINUS communis L 

分 类 号：S565.6[农业科学—作物学]