川芎嗪下调炎症和黏附基因的表达而抑制血管新生内膜的形成  

Tetramethylpyrazine inhibits neointimia formation induced by platelet- derived growth factor through suppressing inflammatory and adhesion molecular expression

作　　者：张红兵[1] 李强[2] 冯靖[3] 狄柯坪[2] 

机构地区：[1]河北经贸大学生物科学与工程学院,石家庄050061 [2]解放军白求恩医务士官学校,石家庄050081 [3]河北化工医药职业技术学院,石家庄050026

出　　处：《中国临床药理学杂志》2014年第4期340-343,共4页The Chinese Journal of Clinical Pharmacology

基　　金：河北省中医药管理局指令性计划基金资助项目(2008082)

摘　　要：目的观察川芎嗪对内皮损伤诱导的新生内膜形成的影响。方法用贴块法,培养血管平滑肌细胞(VSMCs),建立血小板源性生长因子(PDGF)诱导的细胞增殖模型。用细胞计数法观察4个浓度(0,20,40,80μmol·L-1)川芎嗪对VSMCs增殖的影响;用Western blot分析各组细胞中反式作用因子激活蛋白-1(AP-1)、增殖细胞核抗原(PCNA)、环氧合酶-2(COX-2)、细胞间黏附分子-1(ICAM-1)和整合素连接激酶(ILK)等的表达变化;用球囊剥脱法观察对内皮损伤诱导的新生内膜形成的影响。结果川芎嗪呈剂量依赖性地抑制PDGF诱导的细胞增生以及AP-1、PCNA、COX-2、ICAM-1等相关基因的表达,有效阻断黏附分子ILK信号。川芎嗪可预防球囊损伤诱导的血管新生内膜肥厚,明显降低内膜/中膜面积(I/M)比值。结论川芎嗪通过抑制增殖和炎性基因表达、阻断黏附分子信号传递等形式发挥抗血管内膜异常增殖的作用。Objective To investigate the effect of tetramethylpyrazine （ TMP） on neointimal hyperplasia induced by balloon injury.Methods VSMCs were cultured by tissue explants adherent method and VSMCs proliferation models induced by platelet -derived growth factor （ PDGF） were established.Cell counting analysis was adopted to evaluate the in-hibitory effects of TMP in a dose of 0, 20, 40 and 80 μmol ＆#183; L-1 on VSMCs.Western blot analysis was performed to analyze the effect of TMP on the activator protein -1 （ AP-1 ） , proliferating cell nuclear antigen （ PCNA ） , cyclooxygenase （ COX-2 ） , intercellular adhesion molecular （ ICAM -1 ） , and integrin -linked kinase （ ILK ） expressions in VSMCs.Hemetoxylin and Eosin （ HE） staining was performed to investi-gate the effects of TMP on the neointimal hyperplasia induced by balloon injury.Results TMP inhibited cell proliferation induced by PDGF （ 20μg＆#183; L-1 ） in a dose-dependent （0, 20, 40, 80 μmol＆#183; L-1 ） manner.Remarkable decreases of AP -1, PCNA, COX-2, ICAM-1 and ILK expression were demonstrated in the TMP group compared with the model group in VSMCs.TMP inhibited the neointimal thickening induced by ballon injury , with a significantly lower ratio of intima -to-media area （ l/M） in the TMP group.Conclusion TMP plays a role in preventing neointimal hyperplasia through inhibiting or blocking proliferation ,＆amp;nbsp;expressions of inflammatory genes and adhesion molecules.

关 键 词：川芎嗪 血管平滑肌细胞 炎症 黏附 

分 类 号：R285.5[医药卫生—中药学] R076[医药卫生—中医学]