不同表型的结核分枝杆菌诱导U937细胞凋亡率差异分析  

Analysis for the differences of macrophages U937 apoptosis infected by clinical isolates of Mycobacterium tuberculosis with different resistant phenotype

作　　者：胡族琼[1] 谭耀驹[1] 关平[1] 陈虹[1] 孟繁荣[1] 黄艳君[1] 刘燕文[1] 

机构地区：[1]广州市胸科医院,广东广州510095

出　　处：《微生物学免疫学进展》2014年第1期9-12,共4页Progress In Microbiology and Immunology

基　　金：广州市医药卫生科技项目(201102A213131)

摘　　要：目的利用流式细胞技术检测耐多药结核分枝杆菌(Multidrug resistant mycobacterium tuberculosis,MDRTB)临床分离株和结核分枝杆菌一、二线药物敏感株分别感染U937细胞后的凋亡率及其时相性变化。方法用两组细菌分别感染U937细胞,在感染后2、4、8及12 h用流式细胞技术检测各组感染U937细胞的凋亡率,并分析其时相性变化。结果结核分枝杆菌MDR株和敏感株均能诱导U937细胞凋亡,两组细胞凋亡率比较差异有统计学意义(P<0.01),MDR株诱导U937细胞凋亡率显著低于敏感株诱导的凋亡率,且在诱导后2、4、8及12 h各时间点的凋亡率都显著低于敏感株组,诱导早期凋亡率升高显著,特别是诱导后2 h凋亡率升高最显著,4 h以后凋亡率升高逐渐变慢。结论结核分枝杆菌临床分离株感染U937细胞后能快速诱导其凋亡,凋亡率与诱导菌株的耐药表型相关,敏感株诱导的凋亡率显著高于MDR株,诱导早期凋亡率升高最显著,4 h以后凋亡率升高减慢。Objective To compare the apoptosis rate of macrophages U 937 infected with clinical isolates of MDR-TB and susceptible Mycobacterium tuberculosis by using a flow cytometry .Methods Macrophages U937 were respectively infected with MDR-TB and susceptible clinical isolates , then the apoptosis rate of macrophages U 937 were detected by the flow cy-tometry after infection in 2, 4, 8 and 12 hs and the phase changes of apoptosis rate of infected macrophages U 937 were ana-lyzed.Results U937 apoptosis can be induced both by the MDR-TB and the susceptible isolates , but the differences are significant in the apoptosis rates in between two types of isolates （P＆lt;0.01）.The apoptosis rates of U937 infected with MDR-TB are significantly lower than that with susceptible isolates , and all of the apoptosis rates of U 937 after infection in 2, 4, 8 and 12 hs with MDR-TB are significantly lower than that with susceptible isolates .The apoptosis rates of U937 in-crease markedly in early stage of infection , especially after infection in 2 hs.The apoptosis rates slowly rise down after in-fection in 4 hs.Conclusion The macrophage U937 apoptosis rapidly occurrs after infecting with the isolates of mycobacte-rium tuberculosis.The apoptosis rates of U937 are correlated to the isolates of MDR-TB, and are significantly higher in sus-ceptible isolates than these in MDR-TB isolates, but rise slowly down after infection in 4 hs.

关 键 词：结核分枝杆菌 临床分离株 耐多药结核分枝杆菌 巨噬细胞U937 凋亡 

分 类 号：R378.911[医药卫生—病原生物学]