破伤风杆菌产毒培养基主要原材料的替代研究  

The replacement of main materials for culture of Clostridium.tetani in a toxin production medium

作　　者：曹玲[1] 杨海珍[1] 孙一玫[1] 李国晏[1] 谢澎[1] 

机构地区：[1]兰州生物制品研究所有限责任公司甘肃省疫苗工程技术研究中心,甘肃兰州730046

出　　处：《微生物学免疫学进展》2014年第1期31-35,共5页Progress In Microbiology and Immunology

摘　　要：目的探索用干粉状的肝浸粉、胰酪胨、月示胨代替传统破伤风杆菌产毒培养基中使用的猪肝水透析外液、胰酶酪蛋白消化液、浓胨水,以满足规模化生产的需求。方法与传统的破伤风杆菌产毒培养基同时用于破伤风类毒素的生产过程,由小批量生产逐步转化到规模化批量生产,经多批次试验,以检测不同组分制备的培养基各生化指标和接种细菌后培养的产毒水平进行比对。先确定肝浸粉可以取代猪肝水透析外液,在此基础上筛选出代替浓胨水的月示胨,再以待用的胰酪胨配合肝浸粉、月示胨制备多批次不同生产量的破伤风杆菌产毒培养基。结果经过多批次小批量扩大到批量规模化生产的试验,用肝浸粉、胰酪胨、月示胨配制的破伤风杆菌产毒培养基与传统工艺制备的破伤风杆菌产毒培养基相比较,无统计学意义(P>0.05)。结论肝浸粉、胰酪胨、月示胨可以替代传统破伤风杆菌产毒培养基中的猪肝水透析外液、胰酶酪蛋白消化液、浓胨水,适用于破伤风类毒素的规模化生产。Objective To explore using dry powdered liver extract powder , trypticase , and peptone as main materials , it is the need for large-scale production , preparation was carried out for the medium production of tetanus toxin instead of the traditional medium contained liver extracellular fluid , trypsin digestion of casein and concentrated peptone water . Methods The prepared midea and the traditional media were both used in production process of tetanus toxin , from a sim-ple procedure to a large scale gradually , different components of the culture medium were changed by the multi -batch exper-iments and inoculate bacterial seeds , and cultured toxin production levels were obtained and compared , so did for index of biochemical standard .Firstly, on the basis of the replacement of liver extract powder to the extra dialysis fluid , then selec-tion peptone to replace concentrated peptone water was carried out .In combination of these three materials in a toxin pro-duction medium , comparative tests were performed in both of media .Results Several tests are completed , from a small capacity to a production process , the biochemistry indexes are obtained in media after cultivation of bacteria .In preparation of toxin, the medium contained liver extract powder , trypticase and peptone was compared to the traditional medium , it has no statistical significant difference（P〉0.05）in both media.Conclusions The liver extract powder, trypticase, and pep-tone can replace the liver extra-dialysis fluid, trypsin digestion of casein and concentrated peptone water in the traditional medium in preparation of tetanus toxin in an industrial production .

关 键 词：培养基 破伤风梭状芽胞杆菌 原材料 

分 类 号：R378.81[医药卫生—病原生物学]