卤米松、黄芩提取物联合用药对白癜风小鼠模型的影响  

Effect of halometasone in combination with scutellaria baicalensis georgi on the vitiligo mice

作　　者：祝逸平[1] 王遂泉[1] 卢良君[1] 许爱娥[1] 

机构地区：[1]浙江中医药大学附属杭州市第三人民医院,浙江杭州310009

出　　处：《中国药理学通报》2014年第4期554-558,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81071294);省部共建项目(No WKJ2012-2-036);市科委重大专项创新项目(No 20122513A02);浙江省自然科学基金项目(No LY13H11001);省科技厅项目(No 2011C23134);国家临床重点专科建设项目

摘　　要：目的探讨卤米松、黄芩提取物联合用药对莫诺苯宗(monobenzone)诱导的白癜风小鼠模型发生发展的影响。方法应用40%莫诺苯宗乳膏诱导C57BL/6小鼠脱色,建立白癜风小鼠模型;研究卤米松(halometasone)联合黄芩提取物(scutellaria baicalensis georgi)用药对白癜风小鼠的脱色作用;通过肉眼观察小鼠的毛发脱色,反射共聚焦显微镜(reflectance confocal microscopy,RCM)观察皮肤的黑色素和黑素细胞,免疫荧光检测CD8+T细胞,酶联免疫吸附剂测定(enzyme linked immunosorbent assay,ELISA)测定血清中白介素-6(interleukin-6,IL-6)、肿瘤坏死因子-α(tumor necrosis factor,TNF-α)的含量。结果模型组小鼠在用药部位及非用药部位有毛发脱色现象。黄芩组疗效不明显,卤米松组和卤米松联合黄芩组小鼠脱色减少,包括其发生率、出现时间和面积评分较模型组低;且局部CD8+T细胞浸润减少;卤米松联合黄芩组小鼠血清中IL-6、TNF-α的含量减少明显。结论卤米松、黄芩提取物联合用药对白癜风小鼠有一定的作用,且效果优于单独使用卤米松组和黄芩组,可为临床白癜风的治疗提供参考。Aim To study the effect of halometasone in combination with scutellaria baicalensis georgi on the vitiligo mice induced by monobenzone. Methods 40% monobenzone cream was applied to induce vitiligo in C57BL/6 mice. Through the halometasone, halo-metasone and scutellaria baicalensis georgi combined with 40% monobenzone cream, the influence of halo-metasone and scutellaria baicalensis georgi on mice de-colorizing was studied. Hair decolorizing was observed with the naked eye, the skin decolorizing was observed by reflectance confocal microscopy （ RCM ） , and CD8 ＋T cell infiltration was tested with immunofluores-cence detection. The serum levels of interleukin-6（IL-6 ） and tumor necrosis factor-α（ TNF-α） were deter-mined by enzyme linked immunosorbent assay （ ELISA） . Results Mice in model group showed de-pigmentation at both the monobenzone application part and non-application part. The halometasone group did not show significant therapeutic efficacy. In halometa-sone and scutellaria baicalensis georgi treatment group, there was less decolorization, the occurrence ratio, the scores of occurring time and size were lower compared with model group. There were fewer infiltrated lympho-cytes and CD8 ＋T cells. Halometasone and scutellaria baicalensis georgi group also showed that the serum levels of IL-6,TNF-α decreased. Conclusion Halo-metasone and scutellaria baicalensis georgi have thera-peutic effect on vitiligo mice induced by monobenzone.

关 键 词：卤米松 黄芩 白癜风 动物模型 CD8 +T细胞 IL-6 TNF-Α IL-6 TNF-Α 

分 类 号：R332[医药卫生—人体生理学] R284.1[医药卫生—基础医学]