动物模型血栓长度及重量比较探索深静脉血栓形成病变分期  

RESEARCH OF DEEP VENOUS THROMBOSIS STAGE BY COMPARING LENGTH AND WEIGHT OF RAT INFERIOR VENA CAVA THROMBUS

作　　者：白云城[1] 赵学凌[1] 周如丹[1] 周志化[1] 吴雪梅[1] 

机构地区：[1]昆明医科大学第一附属医院骨科,昆明650032

出　　处：《中国修复重建外科杂志》2014年第4期507-510,共4页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家自然科学基金面上项目(81060151);云南省卫生科技项目(2012WS0007);云南省博士研究生学术新人奖资助项目~~

摘　　要：目的 比较大鼠下腔静脉血栓模型不同时间点血栓长度及重量变化情况,探讨血栓开始形成、状态稳定、消退等血栓形成病变分期。方法 取健康雌性SD大鼠48只,体重180～230 g,采用狭窄法阻断下腔静脉大部分血流,制备下腔静脉血栓模型。造模后2、4、8、12、24、48 h及3、7、14 d开腹观察,取材,称量血栓组织长度和重量,并行组织学染色观察。结果 2 h开始有血栓形成,2、4 h血栓长度和重量比较差异无统计学意义（P〉0.05）;6 h开始血栓长度和重量显著上升,6～48 h与2、4 h时比较差异均有统计学意义（P〈0.05）,6～48 h内各时间点间比较差异无统计学意义（P〉0.05）,进入稳定期;造模后3 d血栓开始出现消退,血栓长度、重量较术后48 h均显著降低（P〈0.01）;7 d血栓长度、重量较3 d时进一步降低（P〈0.05）;21 d未观察到血栓,下腔静脉血管再通。HE染色示造模后2 h有血栓形成;6～48 h,随造模时间延长,管腔内充血加剧,并有炎性细胞浸润,以中性粒细胞为主;3 d可观察到血栓机化现象;7 d机化现象进一步加剧;21 d血栓和机化的肉芽组织均已消退。结论 大鼠狭窄法造模结束至造模后6 h是血栓形成的起始期,造模后6～48 h是血栓形成的稳定期,造模后3 d开始进入血栓消退期,至21 d血栓完全消退,下腔静脉再通。Objective To understand the initiation, maturing, and resolution of thrombus by comparing the length and weight of thrombus at different time in the rat inferior vena cava （IVC） stenosis model Methods Forty-eight female Sprague Dawley rats, weighing 180-230 g, were selected to prepare the IVC stenosis model by blocking the most of the IVC blood flow. The length and weight of IVC thrombus were measured at different time points, the histological features were observed via HE staining. Results Blood clots formed after 2 hours of modeling, the thrombus length and weight showed no significant difference between at 2 hours and 4 hours after modeling （P 〉 0.05）. The thrombus length and weight increased significantly at 6 hours, showing significant differences between at 2 and 4 hours and at 6, 8, 12, 24, and 48 hours （P 〈 0.05）; and from 6 to 48 hours, there was no significant difference in the thrombus length and weight （P 〉 0.05）, indicating that thrombus was stable, or maturing. Blood clots began to become smaller after 3 days when compared with ones at 48 hours （P 〈 0.05）, indicating the start of resolution at 3 days. At 7 days, the thrombus length and weight became further smaller when compared with ones at 3 days （P 〈 0.05）. The thrombus completely subsided at 21 days, the IVC recanalized. HE staining showed that thrombus formed after 2 hours of modeling; from 6 to 48 hours, the lumen became hyperemia, and the inflammatory cells, especially neutrophils, could be found. The organization of thrombus could be observed at 3 days and 7 days; thrombus gradually vanished at 21 days. Conclusion The time of thrombus initiation, maturing stage, and resolution stage is 6 hours, 6 to 48 hours, and 3 to 21 days after modeling, respectively.

关 键 词：静脉血栓 血管生物学 动物模型 大鼠 

分 类 号：R543.6[医药卫生—心血管疾病]