低温低碳氮比好氧反硝化菌的筛选及鉴定  

Screening and Identification of an Aerobic Denitrifying Bacterium with Low C/N Ratio at Low Temperature

作　　者：周兰影[1,2] 马秀兰[3] 张晨东[3] 王呈玉[3] 张凤君[1] 

机构地区：[1]吉林大学地下水资源与环境教育部重点实验室,长春130021 [2]吉林省产品质量监督检验院,长春130022 [3]吉林农业大学资源与环境学院,长春130118

出　　处：《科技导报》2014年第11期33-37,共5页Science & Technology Review

基　　金：吉林省重大科技攻关项目(20130204054SF);国家环境保护公益性行业科研专项(201009009)

摘　　要：传统的反硝化工艺存在反硝化细菌的世代时间较长（特别是在低温的冬季）、水力停留时间长、运行和投资费用大等问题。为寻找更好的反硝化细菌，从冬季北方城市污水厂驯化活性污泥中分离出1株耐低温、低碳氮比，且脱氮率较高的好氧反硝化菌株，命名为HFX08。初步鉴定该菌株为假单胞茵属（Pseudomonas），G^-菌。该菌株的最适生长温度为10～20℃，生长曲线符合S型曲线，拟合方程相关性系数达到了差异极显著水平。随着碳氦比的增加，该菌株的反硝化速率随之提高，脱氮率最高可达92％。The conventional denitrification process has limited applications due to its disadvantages, such as long generation time of denitrifying bacteria, especially in cold winter, long hydraulic retention time and high costs. In this study, an aerobic denitrifying bacterium designated as HFX08 was isolated from a domestic sewage plant in a northern city of China in winter. The strain HFX08 exhibited high denitrification performance under low C/N ratio at low temperature. The results indicated that the strain HFX08 was G- and identified as Pseudomonas sp. The optimum temperature for its growth is in the range of 10-20℃. Its growth curve was fitted to S growth curve, whose fitting coefficient reached the level of extremely significant difference. The nitrogen removal rate by strain HFX08 increased with increasing C/N ratio, up to 92%.

关 键 词：好氧反硝化菌 假单胞菌属 脱氮率 碳氮比 低温 

分 类 号：X703[环境科学与工程—环境工程] X172