Box-Behnken响应面法优化芦笋总皂苷超声提取工艺  

Optimization of Ultrasound Extraction Technology for Total Saponins from Asparagus officinalis by Box-Behnken Response Surface Methodology

作　　者：揭晶[1] 赵越[2] 王皓[1] 

机构地区：[1]武汉生物工程学院制药工程系,武汉430415 [2]广东药学院中药学院,广州510006

出　　处：《中国实验方剂学杂志》2014年第8期8-11,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(31302144);湖北省自然科学基金项目(2012FFB05201);2012年度湖北省高等学校青年教师深入企业行动计划项目(XD2012501)

摘　　要：目的:优选芦笋总皂苷的超声提取工艺。方法:以总皂苷含量为指标,在单因素试验基础上,通过Box-Behnken响应面法考察乙醇体积分数、料液比、超声时间和温度对提取工艺的影响。结果:最佳超声提取工艺为加15倍量74%乙醇于50℃超声提取54 min;总皂苷平均质量分数13.059%(RSD 1.63%),与预测值(13.185%)的偏差较小。结论:优选的提取工艺稳定可行,为芦笋总皂苷的开发应用提供实验依据。Objective：To optimize ultrasound extraction technology of total saponins from Asparagus officinalis.Method：With the content of total saponins as index,effects of ethanol concentration,solid-liquid ratio,ultrasonic time and temperature on extraction technology were investigated by single factor tests and Box-Behnken response surface methodology.Result：Optimum ultrasound extraction technology was as following：ultrasonic extracted 54 min with 15 times the amount of 74％ ethanol at 50 ℃ ; Under these conditions,the average mass fraction of total saponins was 13.059％,which was wery close to the predicted value of 13.185％.Conclusion：This optimized technology was stable and feasible to provide a basis for development and application of total saponins in A.officinalis.

关 键 词：芦笋 总皂苷 超声提取工艺 单因素试验 Box-Behnken响应面法 

分 类 号：R284.2[医药卫生—中药学] R283.6[医药卫生—中医学]