复方天麻钩藤口腔崩解片的半仿生提取工艺优选  

Optimization of Semi-bionic Extraction Technology for Compound Tianma Gouteng Orally Disintegrating Tablets

作　　者：李钦青[1] 郭蕾[1] 柴金苗[1] 张俊龙[1] 

机构地区：[1]山西中医学院,太原030024

出　　处：《中国实验方剂学杂志》2014年第8期12-15,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30873236);太原市科技项目(11014918)

摘　　要：目的:优选复方天麻钩藤口腔崩解片的半仿生提取工艺。方法:以天麻素、钩藤总碱、芍药苷和阿魏酸提取量的综合评分为指标,采用正交试验考察加水量、提取时间、提取温度对提取工艺的影响。采用HPLC测定天麻素、芍药苷和阿魏酸含量,流动相分别为乙腈-0.05%磷酸溶液(3∶97),乙腈-0.1%磷酸溶液(14∶86),乙腈-0.1%磷酸溶液(17∶83),检测波长分别为220,230,316 nm,运用UV测定钩藤总碱含量。结果:最佳提取工艺为加12倍量水于60℃提取2 h;5倍处方量药材中天麻素、钩藤总碱、芍药苷、阿魏酸提出总量分别为0.479,1.276,4.802,0.061 g。结论:优选的工艺条件稳定可行,适合复方天麻钩藤口腔崩解片的工业化生产。Objective：To optimize semi-bionic extraction technology of compound Tianma Gouteng orally disintegrating tablets.Method：With composite score of gastrodin,rhomotoxine,paeoniflorin and ferulic acid extraction amounts as index,L9 （34） orthogonal test was adopted to investigate effects of the amount of water,extracting time and temperature on semi-bionic extraction technology.HPLC was used to determine contents of gastrodin,paeoniflorin and ferulic acid,mobile phase were acetonitrile-0.05％ phosphoric acid （3∶97）,acetonitrile-0.1％ phosphoric acid （14 ∶ 86） and acetonitrile-0.1％ phosphoric acid （17 ∶ 83）,detection wavelength were 220,230 and 316 nm,respectively; The content of rhomotoxine was determined by UV.Result：Optimum extraction technology was as follows：extracted 2 h with 12 times the amount of water at 60 ℃ ;Total extraction amounts of gastrodin,rhomotoxine,paeoniflorin and ferulic acid in five times the amount of prescriptions medicines were 0.479,1.276,4.802 and 0.061 g,respectively.Conclusion：This optimized extraction process was stable and feasible,which was suitable for industrial production of compound Tianma Gouteng orally disintegrating tablets.

关 键 词：正交设计 口腔崩解片 半仿生提取法 高效液相色谱法 紫外分光光度法 

分 类 号：R283.6[医药卫生—中药学] R284.2[医药卫生—中医学]