星点设计-响应面法优选枳实总黄酮的大孔吸附树脂纯化工艺  

Optimization of Purification Process for Total Flavonoids in Aurantii Fructus Immaturus with Macroporous Resin by Central Composite Design-Response Surface Methodology

作　　者：马丽[1] 陈家仪[1] 陈稚[1] 梁巧文 曹骋[1] 曾元儿[1] 

机构地区：[1]广州中医药大学,广州510006

出　　处：《中国实验方剂学杂志》2014年第8期33-36,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广东省大学生创新实验项目(1057211030)

摘　　要：目的:优选枳实总黄酮的大孔树脂纯化工艺。方法:选择吸附时间、乙醇体积分数、洗脱用量为自变量,总黄酮吸附-洗脱率为因变量,在单因素试验基础上,采用星点设计-响应面法优选大孔树脂纯化工艺。结果:选择AB-8型大孔树脂,最佳纯化工艺为上样液质量浓度3.5 g·L-1,pH 6.0,上样量0.6 BV,上样速率1 BV·h-1,水洗用量1 BV,洗脱速率2 BV·h-1,吸附时间77 min,乙醇体积分数88%,洗脱剂用量2.4 BV;枳实总黄酮吸附-洗脱率达93.0%,与预测值(92.4%)偏差较小。结论:采用星点设计-响应面法优选的大孔树脂纯化工艺条件稳定可行,预测性好。Objective：To optimize purification process of total flavonoids in Aurantii Fructus Immaturus by macroporous resin.Method：Independent variables were adsorption time,ethanol concentration and eluent volume,while dependent variables was adsorption-elution rate of total flavonoids,central composite designresponse surface methodology was adopted to optimize purification technology on the basis of single factor tests.Result：AB-8 macroporous resin was selected,optimum purification process were as follows：added 0.6 BV of sample solution with concentration of 3.5 g ·L-1 and pH 6.0,sample rate 1 BV ·h-1,adsorption time 77 min,water consumption 1 BV,elution rate of 2 BV ·h-1,eluted with 2.4 BV of 88％ ethanol.Adsorption-elution rate of total flavonoids was up to 93.0％,which had a small deviation with the predicted value of 92.4％.Conclusion：Optimized purification technology was stable and feasible with high predictability.

关 键 词：枳实 总黄酮 星点设计-效应面法 大孔吸附树脂 纯化工艺 单因素试验 

分 类 号：R284.2[医药卫生—中药学] R283.6[医药卫生—中医学]