UPLC测定参麦注射液中人参皂苷Rg_1、人参皂苷Re、人参皂苷Rb_1的含量  

Content Determination of Ginsenoside Rg_1,Ginsenoside Re and Ginsenoside Rb_1 in Shenmai Injection by UPLC

作　　者：林苏娜[1] 余楚钦[1] 林华庆[1] 郭玉海[2] 林的仕[1] 郑文忠[1] 黄晓敏[1] 

机构地区：[1]广东药学院药物研究所,广州510006 [2]广东省中医院,广州510006

出　　处：《中国实验方剂学杂志》2014年第8期48-50,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81271625)

摘　　要：目的：采用超高效液相色谱法分离测定参麦注射液中人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1的含量.方法：采用ACQUITY（R）UPLC HSS T3（2.1 mm×50 mm,1.8 μm）色谱柱,流动相乙腈（A）-0.05％磷酸（B）,梯度洗脱（0 ～5 min,19％A;5～12 min,19％ ～ 28％A;12 ～ 18 min,28％～40％A）,流速0.3 mL· min-,检测波长203 nm,柱温25℃.结果：人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1分别在0.020 96～0.209 6,0.017 64 ～0.176 4,0.02388～0.2388 g·L-1与相应峰面积呈良好线性关系,平均加样回收率（n=6）分别为100.12％,100.08％,99.51％,RSD分别为1.60％,2.03％,1.15％.结论：与HPLC相比,UPLC在不影响分离效果的情况下可显著提高参麦注射液中人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1的分析速度,改善分析效果,同时可减少溶剂的消耗.该方法简便、快捷、准确、重复性好,可代替HPLC法用于参麦注射液的多指标质量控制.Objective：To establish a method to determine the content of ginsenoside Rg1,ginsenoside Re and ginsenoside Rb1 in Shenmai injection by ultra performance liquid chromatography （UPLC）.Method：ACQUITY （R） UPLC HSS T3 column （2.1 mm× 50 mm,1.8 μm） was used with mobile phase consisted of acetonitrile （A）-0.05％ phosphoric acid solution （B） by gradient elution （0-5 min,19％ A ; 5-12 min,19％-28％A; 12-18 min,28％-40％ A） at a flow rate of 0.3 mL ·min-1; the wavelength of detector was 203 nm.Result：Ginsenoside Rg1,ginsenoside Re and ginsenoside Rb1 had good linearity within the range of 0.020 96-0.209 6,0.017 64-0.176 4,0.023 88-0.238 8 g·L-1 respectively.The average recoveries （n =6） were 100.12％,100.08％,99.51％ and RSDs were 1.60％,2.03％,1.15％ respectively.Conclusion：UPLC method may greatly improve the separation efficiency and analysis speed in the case of ginsenoside Rg1,ginsenoside Re and ginsenoside Rb1 in Shenmai injection while reducing the solvent consumption.As an alternative of conventional HPLC,UPLC is more convenient,rapid,accurate and excellent repeatability.

关 键 词：超高效液相色谱法 参麦注射液 人参皂苷Rg1 人参皂苷RE 人参皂苷RB1 

分 类 号：R284.1[医药卫生—中药学]