不同生长年限山豆根中苦参碱和氧化苦参碱的含量比较  

Comparison of Matrine and Oxymatrine in Radix Sophorae Subprostrata of Different Growing Years

作　　者：彭红华[1] 蒋嫦月 林吴[1] 田辉[1] 杨东爱[2] 张晶晶[1] 员晓云[1] 梁国隆[1] 梁臣艳[1] 

机构地区：[1]广西中医药大学,南宁530001 [2]广西民族医药研究所,南宁530001

出　　处：《中国实验方剂学杂志》2014年第8期72-75,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西教育厅科研立项课题(200810LX101);广西自然科学基金创新团队研究项目(2011GXNSFF018006)

摘　　要：目的：探讨不同生长年限山豆根中苦参碱和氧化苦参碱的含量,为建立和完善中药材山豆根的质量控制体系和适宜采收时间提供实验依据.方法：采用HPLC测定,色谱柱welch materials XB-C18,流动相乙腈-0.08％三乙胺水溶液（12∶88）,流速0.8 mL· min-,检测波长220 nm,检测温度25℃.结果：不同生长年限的山豆根中生物碱的含量差异明显.1,2年生山豆根中生物碱的含量较低,3年生的药材含量明显升高,3～6年的药材含量变化不大.结论：苦参碱和氧化苦参碱的含量变化规律可为确定山豆根的采收年限提供参考依据.Objective：To study the content of matrine and oxymatrine in Radix Sophorae Subprostrata of different growing years.Method：HPLC was adopted,the separation was performed on a welch materials XB-C18 column,acetonitrile-0.08％ triethylamine （12∶88） was used as the mobile phase,the flow rate was 0.8 mL ·min-1,detection wavelength was set at 220 nm with temperature at 25 ℃.Result：The diversity of the content of matrine and oxymatrine in Radix Sophorae Subprostrata of different growing years was great.The ingredients in 1-2 years of Radix Sophorae Subprostrata were in the lower levels,the ingredients in 3 years increased significantly and the ingredients in over 3 years had stable change.Conclusion：The changing rules of matrine and oxymatrine provide scientific basis for the collecting years.

关 键 词：山豆根 苦参碱 氧化苦参碱 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]