人参皂苷Rb_1与罗格列酮联合给药对2型糖尿病记忆障碍小鼠海马胰岛素信号传导通路的影响  

Effect and Mechanism of Ginsenosides Rb_1 on Insulin Signaling Pathway in Type 2 Diabetes Mice with Memory Impairment

作　　者：高晓斐[1] 毛黎黎 孙子微[1] 杨彩虹[1] 岳仁宋[2] 

机构地区：[1]成都中医药大学,成都610072 [2]成都中医药大学附属医院,成都610072

出　　处：《中国实验方剂学杂志》2014年第8期137-141,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81072775)

摘　　要：目的:研究人参皂苷Rb1对2型糖尿病小鼠记忆功能及胰岛素信号传导通路的影响及作用机制。方法:C57BL小鼠为正常对照组,Kkay小鼠造模成功后根据血糖水平随机分为模型对照组、阳性组和复合给药高、中、低剂量组。阳性组用马来酸罗格列酮片(0.003 g·kg-1)ig,复合高、中、低剂量组分别用马来酸罗格列酮片(0.003 g·kg-1)ig和人参皂苷Rb1(0.06,0.03,0.015 g·kg-1)ig,其余用等体积生理盐水ig 6周。给药前、后各行1次Morris水迷宫实验;免疫组化方法检测大脑海马中1-磷脂酰肌醇-3激酶(PI-3K)水平;蛋白印迹法(Western blotting)检测糖原合酶激酶-3β(GSK-3β)、Tau蛋白及Tau蛋白磷酸化(p-Tau)和胰岛素受体(INS R)水平。结果:与正常组相比,模型组平台潜伏期明显升高(P<0.05),PI-3K活性明显偏低(P<0.05),GSK-3β和p-Tau水平均明显偏高(P<0.05);与模型组相比,罗格列酮组与之各项指标差异均不明显,复合给药组平台潜伏期均明显降低(P<0.05),PI-3K活性均明显升高(P<0.05),GSK-3β和p-Tau水平均明显降低(P<0.05);给药后各组间胰岛素受体水平差异无统计学意义。结论:在常规降糖的基础上,人参皂苷Rb1对2型糖尿病痴呆小鼠记忆水平有明显改善作用,其可能的作用机制为激活PI-3K蛋白、抑制GSK-3β表达、减轻Tau蛋白过度磷酸化,进而改善大脑海马胰岛素信号传导通路,阻止、延缓其相关神经病理进程。Objective：To study the effect and mechanism of ginsenosides Rb1 on memory impairment of type 2 diabetes.Method：C57BL mice were as normal control group and Kkay mice with high blood sugar as model 1 group.The model mice were divided into positive group and composite group with high,middle and low dose.The positive group was treated with Rosiglitazone maleate tablets （0.003 g·kg-1） ig,and composite group was given the same Rosiglitazone tablets and ginsenosides Rb1 （0.06,0.03,0.015 g ·kg-1） ig.After Morris water mazeimmunohistochemistry was used to assay 1-phosphatidy linosital 3-kinase （PI-3K） /Akt,and Western blotting was used to detect glycogen synthase kinase-3β GSK-3β,and p-Tau and insulin receptor （INSR） level.Result：INSR levels were almost the same in every group.Compared with normal control group,model group had more active PI-3K/Akt and lower GSK-3β and p-Tau level.Howere,compared with control group,there was no significant differences in positive group,while PI-3K/Akt was more active,and GSK-3β and p-Tau was contrary in composite group.Conclusion：Ginsenosides Rb1 can improve the memory of type 2 diabetes mice,the possible mechanism may be related to improving insulin signaling pathway.

关 键 词：人参皂苷 糖尿病性记忆障碍 糖原合酶激酶-3Β Tau蛋白 磷脂酰肌醇-3激酶 胰岛素信号传导通路 

分 类 号：R285.5[医药卫生—中药学]