S Phase Cell Percentage Normalized BrdU Incorporation Rate, a New Parameter for Determining S Arrest  

作　　者：ZHAO Peng FU Juan Ling YAO Bi Yun JIA Yong Rui ZHOU Zong Can 

机构地区：[1]Department of Toxicology,Peking University Health Science Center [2]Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety,Peking University Health Science Center [3]Medical and Healthy Analytical Center,Peking University Health Science Center

出　　处：《Biomedical and Environmental Sciences》2014年第3期215-219,共5页生物医学与环境科学（英文版）

基　　金：supported by the National Nature Science Foundation of China(Nos.81370079 and 81001253);the Beijing Natural Science Foundation(No.7132122)

摘　　要：In this study, a new parameter, S phase cell percentage （S fraction） normalized BrdU （SFN-BrdU） incorporation rate, was introduced to detect $ arrest. The results showed a positive linear correlation between the BrdU incorporation rate and the S fraction in unperturbed 16HBE cells. Theoretical analysis indicated that only S arrest could result in a decrease in the SFN-BrdU incorporation rate. Additionally, the decrease in SFN-BrdU incorporation rate and the activation of DNA damage checkpoints further demonstrated that S arrest was induced by diethyl sulfate treatment of 16HBE cells. In conclusion, $FN-BrdU incorporation rate can be used to detecting S arrest.In this study, a new parameter, S phase cell percentage （S fraction） normalized BrdU （SFN-BrdU） incorporation rate, was introduced to detect $ arrest. The results showed a positive linear correlation between the BrdU incorporation rate and the S fraction in unperturbed 16HBE cells. Theoretical analysis indicated that only S arrest could result in a decrease in the SFN-BrdU incorporation rate. Additionally, the decrease in SFN-BrdU incorporation rate and the activation of DNA damage checkpoints further demonstrated that S arrest was induced by diethyl sulfate treatment of 16HBE cells. In conclusion, $FN-BrdU incorporation rate can be used to detecting S arrest.

关 键 词：BRDU RATE S Phase Cell Percentage Normalized BrdU Incorporation Rate a New Parameter for Determining S Arrest 

分 类 号：R114[医药卫生—卫生毒理学]