桃红四物汤对肝纤维化大鼠肝组织星状细胞增殖的影响  

Effect of Taohongsiwu Decoction on the Proliferation of Hepatic Stellate Cell in Rats with Hepatic Fibrosis

作　　者：罗利琼[1] 陈东波[2] 

机构地区：[1]广东药学院生命科学与生物制药学院 [2]广东药学院基础学院,广东广州510006

出　　处：《宜春学院学报》2014年第3期69-71,76,共4页Journal of Yichun University

摘　　要：目的:观察桃红四物汤对肝纤维化大鼠肝组织星状细胞(HSC)增殖的影响,探讨其抗肝纤维化作用。方法:将大鼠随机分成4组,每组10只:对照组,模型组,桃红四物汤低、高剂量组。除正常组外,其余各组皮下注射四氯化碳(CCl4)制备肝纤维化大鼠模型,低、高剂量桃红四物汤组灌胃相应剂量桃红四物汤水溶液。检测各组大鼠血清丙氨酸氨基转移酶(GPT)、天冬氨酸氨基转移酶(GOT)、总胆红素(Bil)、总蛋白(TP)、白蛋白(Alb)等肝功能生化指标以及肝星状细胞(HSC)增殖与凋亡,并观察肝组织形态学变化。结果:与模型组比较,桃红四物汤组大鼠血清GPT、GOT、Bil水平明显降低(P<0.05或P<0.01),血清TP、ALB水平明显升高(P<0.05),HSC凋亡率明显提高(P<0.05或P<0.01),而HSC处于G0/G1期细胞比例明显增多(P<0.05或P<0.01),实验性肝纤维化组织形态学得到改善。结论:桃红四物汤能明显改善CCl4致肝纤维化大鼠基础生化指标,延缓纤维化进程,其作用机制可能与促进HSC凋亡并抑制HSC的增殖有关。Objective： To observe the impact of Taohongsiwu decoction on the proliferation of hepatic steUate cell （HSC） in rats with hepatic fibrosis, and explore its anti - hepatic fibrosis effect. Methods： The rats were randomly divided into 4 groups as the control group, the model group, the group with low dose of Taohongsiwu decoction and the group with high dose of Taohongsiwu decoction with 10 rats in each group. Rats in all groups except the normal group were injected with CC14 to prepare the rat model of hepatic fibrosis. Corresponding doses of Taohong- siwu decoction water solution were administrated in rats of the low dose group and the high dose group through intragastric administration. Biochemical indexes of hepatic function of rats in each group were determined including serum alanine aminotransferase （GPT）, aspartate arninotransferase （GOT）, total bilirubin （Bil）, total protein （TP） and albumin （Alb） . Detection of the proliferation and apoptosis of HSC was also performed and the morpho/ogical changes of hepatic tissue were observed. Results： Compared with the model group, rats in the Taohongsiwu decoction group showed obviously lower serum levels of GPT, GOT and Bil （P 〈0. 05 or P 〈0. 01 ） , evidently higher levels of TT and ALB （ P 〈 0. 05）, obviously enhanced apoptosis rate of HSC （ P 〈 0. 05 or P 〈 0. 01 ）, and evidently increased percentage of HSC in the G0/G1 phase. Histomorpho/ogy improvement was achieved in the experimental hepatic fibrosis. Conclusions： Taohongsiwu decoction greatly improves the basic biochemical indexes of rats with CCL4 caused hepatic fibrosis and postpones fibrosis development, and its function mechanism may be associated with the acceleration of HSC apoptosis and inhibition of HSC proliferation.

关 键 词：桃红四物汤 肝纤维化 肝功能 血清指标 

分 类 号：R285[医药卫生—中药学]