α-细辛醚诱导人食管癌细胞系Eca-109凋亡的实验研究  

Experimental Study on α-Asarone Induce Apoptosis in Human Esophagral Carcinoma Eca-109 Cell Line

作　　者：王甜甜[1] 吴芳[1] 朱艳琴[1] 付强[1] 高爱社[1] 

机构地区：[1]河南中医学院,河南郑州450046

出　　处：《中医学报》2014年第4期473-475,共3页Acta Chinese Medicine

摘　　要：目的:探讨α-细辛醚对人食管癌Eca-109细胞凋亡的影响。方法:体外培养人食管癌细胞株(Eca-109),分别给以不同浓度的α-细辛醚对Eca-109细胞进行干预。DAPI染色倒置荧光显微镜观察细胞形态变化,MTT检测细胞增殖率,流式细胞仪Annexin V-PI双染检测细胞凋亡率的变化。结果:不同浓度、不同作用时间的α-细辛醚组细胞增殖抑制率比较,差异有显著性(P<0.05);细胞凋亡指数与阴性对照组比较,差异具有显著性(P<0.05)。结论:α-细辛醚对人食管癌Eca-109细胞凋亡有促进作用。Objective ：To investigate the effect of a-Asarone on the apoptosis of human esophageal carcinoma Eca-109 cell line. Methods： Different concentrations of α-Asarone were used to intervene in the culture of human esophageal carcinoma ceils 109 in vitro. Morphologic changs were viewed under a inverted fluorescence microscope with DAPI staining assay, cell proliferation rate was observed by MTI assay, and cell apoptosis was overviewed by flow cytometry with annexin V-FITC/PI staining. Results： Cell proliferation inhibition rate of Eca- 109 ceils between different-concentration and different-action-time α-Asarone was different （P 〈 0. 05 ） ; cell apoptosis index compared with negative control group was different （P 〈 0.05）. Conclusion ：α-Asarone has a role in promoting human Eca-109 cell apoptosis.

关 键 词：Α-细辛醚 食管癌 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]