柴芩合剂提取工艺研究  

Research on Extraction Technology of Chaiqin Mixture

作　　者：张璐[1] 仇继玺[2] 刘瑞新[1] 

机构地区：[1]河南中医学院第一附属医院,河南郑州450000 [2]河南中医学院,河南郑州450046

出　　处：《中医学报》2014年第4期540-542,共3页Acta Chinese Medicine

基　　金：国家自然科学基金青年基金项目(编号:81001646)

摘　　要：目的:优选柴芩合剂的最佳提取工艺条件。方法:以黄芩苷含量为指标,采用L9(34)正交设计法,对影响其提取率的重要因素(A)加水量、(B)煎煮时间、(C)煎煮次数进行优选。采用HPLC测定黄芩苷的含量,Agilent XDB-C18色谱柱(4.6 mm×150.0 mm,5μm),流动相甲醇-水-磷酸(47:53:0.2),检测波长278 nm,流速1.0 mL,·min-1。结果:优选的最佳水提取工艺条件为6倍量水,提取3次,每次1.5 h。结论:优选出的提取工艺操作简单,生产成本低,符合工业生产的需要。Objective ： To optimize the best extraction technology of Chaiqin Mixture. Methods ： The L9 （ 34 ） orthogonal design method was employed to study the best extracted technics through analyzing the content of baiealin by HPLC with water volume,decocting times and deeoeting time as factors, The determination was carried out on a Agilent XDB-C18 eolume （4.6ram 150mm, 5 pm ） , methanol- water-phosphoric acid （47：53：0.2） as mobile phase with flow rate of 1.0 mt/min, UV detection wavelength was 278 nm. Results： The best optimal water extraction technology conditions for optimization was as follows ： extracted 3 times with 6 times the amount of water, 1.5 h per time. Conclusion ： The optimized extraction technology is simple with low cost,it is suitable for industrial production.

关 键 词：柴芩合剂 黄芩苷 高效液相色谱法 正交试验 

分 类 号：R284.2[医药卫生—中药学]