降钙素基因相关肽对大鼠心室肌细胞膜ATP敏感性钾离子通道的影响  

The effects of calcitonin gene-related peptide on ATP-sensitive potassium channels in rats ventricular cardiomyocytes

作　　者：王鑫[1] 原大江[2] 郭政[2] 

机构地区：[1]山西医科大学,太原市030001 [2]山西医科大学第二医院麻醉科

出　　处：《临床麻醉学杂志》2014年第4期396-399,共4页Journal of Clinical Anesthesiology

摘　　要：目的探讨降钙素基因相关肽(CGRP)与大鼠心室肌细胞膜ATP敏感性钾通道(KATP)之间的关系及其信号转导通路。方法酶解法急性分离SD大鼠心室肌细胞,采用Langendroff灌流装置,分别用含0.1nmol/L CGRP、1nmol/LCGRP8-37(CGRP特异性阻滞剂)、1μmol/L H-89(蛋白激酶A阻滞剂)的细胞外液灌流心室肌细胞,每个细胞采用给药前后自身对照,标准的全细胞膜片钳技术记录KATP电流,待细胞电流稳定后,观察不同药物对KATP电流的影响。结果 0.1nmol/L CGRP对KATP电流有明显的激活作用,电流密度-电压(I-V)曲线明显上移(P<0.05)。1nmol/L CGRP8-37和1μmol/L H-89可使CGRP激活的KATP电流明显降低,I-V曲线明显下移(P<0.05)。结论心肌细胞上CGRP与其特异性受体结合,通过蛋白激酶A激活KATP,增强KATP电流。Objective To observe the relationship between calcitonin gene related peptide （CGRP） and ATP-sensitive potassium channels（K ATP ）as well as to explore the underlying possible signaling pathway. Methods KATe currents on the single ventricular cardiomyocyte obtained from Sprague-Dawley rat byacute enzymatic dissociation was measured with whole-cell patch clamp technique. 0. 1 nmol/L CGRP, 1 nmol/L CGRPs-37 and 1 9mol/L H-89 were added into the suspension of the myocytes. The data were collected after the cell currents stability. The KATP current were used to compare the results by setting a self-control group before and after the administration of drugs. Results The KATe currents density perfusion with 0. 1 nmol/L CGRP was significantly increased （P 〈0. 05）, I-V curve up. Compared with CGRP, the KATe currents density perfusion with 1 nmol/L CGRP8-37 and 1 umol/L H-89 were significantly decreased （P 〈0.05）, I-V curve down. Conclusion With the combination of corresponding specific receptors on the ventricular cardiomyocyte, CGRP could activate KATP channels and increase KATPcurrents via protein kinase A.

关 键 词：降钙素基因相关肽 ATP敏感性钾通道 心肌细胞 全细胞膜片钳 蛋白激酶A 

分 类 号：R614[医药卫生—麻醉学]